%A 凌杏元,周培疆,关和新,景润春,朱英国 %T 运用AFLP技术筛选分离野败型水稻mtDNA中与雄性不育性状相关的片段 %0 Journal Article %D 1999 %J 遗传 %R %P 33-665 %V 21 %N 2 %U {http://www.chinagene.cn/CN/abstract/article_7.shtml} %8 1999-04-10 %X 对野败型水稻不育系(珍汕97A)、保持系(珍汕97B)和F1杂种(汕优63)线粒体DNA进行了AFLP比较,从M/PA引物对选择扩增产物中找到了不育系与保持系差异条带ZA1、ZA2和ZA3。Northern分析表明,片段ZA1黄化苗期无转录产物, 可能是非编码序列;而ZA2、ZA3两片段在不育系、保持系和F1杂种中的转录则有差异,其中片段ZA2在黄化苗期保持系转录,不育系和F1杂种无转录产物;片段ZA3在黄化苗期保持系和F1杂种转录,表明ZA3转录受核恢复基因Rf影响。
Abstract Mitochondrial DNA extracted from Zhenshan 97A、Zhenshan 97B and Shanyou 63(F1) were analysed and compared each other with AFLP technique. Three polymorphismic DNA fragments, ZA1、ZA2and ZA3were found when the mtDNA fragements were amplified with selective primer M/PA. The results of northern analysis show that ZA1 may be the sequence without information, but ZA2、ZA3are the coding sequence. ZA2transcripts only in maintain line, no transcription product was detected in Zhenshan 97A and Shanyou63. ZA3transcripts both in Zhenshan 97B and Shanyou 63(F1), the result indicates that the transcription of ZA3was effected by the nuclear geneRf.