利用谱系追踪方法探究Lgr5在胰腺组织及其类器官中的表达

doi:10.16288/j.yczz.22-022

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Hereditas(Beijing) ›› 2022, Vol. 44 ›› Issue (5): 432-441.doi: 10.16288/j.yczz.22-022

• Research Article • Previous Articles Next Articles

Study on Lgr5 expression in pancreas tissues and organoids by lineage tracing

Bingru Yan¹(), Xianhui Ai¹, Miao Liu¹, Lihong Tian², Yang Liang¹, Chun-Bo Teng¹()

1. College of Life Science, Northeast Forestry University, Harbin 150040, China
2. College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China

Received:2022-01-26 Revised:2022-03-26 Online:2022-05-20 Published:2022-04-18
Contact: Teng Chun-Bo E-mail:heylr5553@163.com;chunboteng@nefu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(32072801)

Abstract

Abstract:

Leucine rich repeat containing G protein-coupled receptor 5(Lgr5) is widely expressed in multiple tissues and can be used as a stem cell marker in a variety of epithelial organs (including the small intestine, colon, stomach and hair follicles). In this study, we used Lgr5-Cre^ERT2+/- and Rosa26-mTmG hybridized transgenic mice to investigate the expression of Lgr5 in both ductal epithelial cells during pancreas development and in vitro cultured pancreatic duct organoids. After induction with Tamoxifen, the Lgr5 expression was analyzed by detecting the enhanced green fluorescence protein in the pancreatic tissue sections in adult animals and embryos at different developmental stages. The results showed that Lgr5 expression was detected neither in adult pancreatic duct epithelia nor in the embryonic pancreatic tissues at day 15.5 or in newborn mice. However, when 4-hydroxy-Tamoxifen was supplemented to the culture medium, EGFP could be detected in the primary pancreatic duct organoids from Lgr5-Cre ^ERT2+/-; Rosa26-mTmG mice. These results suggested that Lgr5 was not expressed in adult and embryonic pancreatic tissues; but could be expressed in the cultured pancreas ductal organoids. The research lays the foundation for exploring specific gene expression patterns in stem/progenitor cells during pancreatic development.

Key words: pancreas, duct, Lgr5, organoid, lineage tracing

Bingru Yan, Xianhui Ai, Miao Liu, Lihong Tian, Yang Liang, Chun-Bo Teng. Study on Lgr5 expression in pancreas tissues and organoids by lineage tracing[J]. Hereditas(Beijing), 2022, 44(5): 432-441.

Figures/Tables 8

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基因名称	引物序列(5′→3′)
GAPDH	F：CACGGCAAATTCAACGGCACAGTCAAGG
GAPDH	R：GTTCACACCCATCACAAACATGG
Cre	F：GCCTGCATTACCGGTCGATGC
Cre	R：TCCCACCGTCAGTACGTGAGATA
mTmG	F：CTCTGCTGCCTCCTGGCTTCT
	R1：CGAGGCGGATCACAAGCAATA
	R2：TCAATGGGCGGGGGTCGTT
Lgr5	F：CTGCTCTCTGCTCCCAGTCT
	R1：ATACCCCATCCCTTTTGAGC
	R2：GAACTTCAGGGTCAGCTTGC

Study on Lgr5 expression in pancreas tissues and organoids by lineage tracing

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