Abstract: Progress in genome research has made it possible to develop new SSR markers by bioinformatics in a relatively narrow region of genome. To realize it, the first thing is to search for potential SSR sites. Any known methods have more or less defects. Efforts were made to develop a local SSR sites searching software in this study. The resultant software, SSRHunter, could accomplish this task perfectly. Furthermore, SSRHunter could provide automatic pretreatment of sequences, routine arrangement of sequences, sequence transformation and convenient report output.