Abstract: To study the expression of differential methylation - associated Staufen gene in colorectal cancer(CRC), we detected the expression of Staufen gene in adenocarcinoma, mucosa adjacent to colorectal cancer（MACC）and corresponding distant normal tissue using RT-PCR and immunohistochemistry. We found that the expression of Staufen gene in MACC and adenocarcinoma of CRC was significantly lower than that in corresponding distal normal tissue at mRNA level(P<0.05.No significant associations were found between age, sex, location of cancer (colon/ rectal), degree of differentiation and metastasis of lymph node with expression of Staufen gene. Lower expression of Staufen protein in MACC was verified by comparing with matched normal tissue and adenocarcinoma（P<0.05）. This study indicated low expression of Staufen gene in colorectal cancer and the Staufen gene might be involved in the development of colorectal cancer.