Abstract: It has achieved great success in plant heterosis by using cytoplasmic male sterility and fertility restorer (CMS/Rf) system. In order to elucidate the action mechanism of Rf gene in this system, many researchers have been contributing themselves to map and clone fertility restorer genes in recent years. Four Rf genes were recently cloned successfully. Here we presented the overview of plant CMS fertility restorer gene mapping, cloning and its molecular mechanism. It was also discussed on utilization of fertility restorer genes in the molecular breeding in plants.