RH (radiation hybrid) has proved to be an effective method in constructing human genome maps (including ESTs, STSs and microsatellites). In this study, based on the information of five human genes (FMR1, IDS, FATE, BGN, F8A) on the X chromosome, the linkage relationship of these five genes in pigs were analyzed by a panel of 96 radiation hybrid cell lines. The results showed that FMR1, IDS, FATE, BGN, F8A were in the same linkage group, when LOD was set at 4. When LOD was set at 5, FMR1 and IDS were in the one group, FATE and BGN in the other group, and F8A was in a group by itself.