Abstract: Abstract: We amplified genomic DNA of the Paramisgurnus dabryanus using the DM degenerate primers and detected a band, approximately 150 bp. After cloned into pMD18-T vector and sequenced, three sequences showed high homology with the DM domain. They were named as PdDmrt1, PdDmrt3, and PdDmrt5. Based on similarities of amino acid se-quences of the DM domain, Dmrt gene protein sequences from six vertebrates species were included in a phylogenetic tree.