Abstract:

Abstract: Gene PsPIP1 (GenBank accession No. EU626398) containing a complete ORF was obtained using rapid ampli-fication of cDNA ends (RACE) from the cDNA library of Polygonum sibiricum Laxm. leaves. The length of cDNA was 1 004 bp, which encoded a peptide of 285 amino acid residues. Based on other kinds of plant aquaporin amino acid sequences, the phylogenetic evolution, and tertiary structure of protein comparison, this gene was classified into aquaporin subfamily. Expression analysis by Real-time PCR showed that PsPIP1 gene was expressed in leaves, stems, and underground stems. The expression level of PsPIP1 gene was higher in leaves than in underground stems and was the lowest in stems. The ex-pression pattern of PsPIP1 gene induced by NaHCO₃ stress and de-stressing also varied remarkably.