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HEREDITAS ›› 2008, Vol. 30 ›› Issue (2): 175-178.doi: 10.3724/SP.J.1005.2008.00175

• 研究报告 • Previous Articles     Next Articles

Molecular cloning and mutation site analysis of AMPD1 gene in swine

LIU Hui1, XU Yao1, ZHAO Li-Li1, YANG Xiu-Qin1, LIU Di1,2   

  1. 1. College of Animal Science Technology, Northeast Agricultural University, Harbin 150030, China; 2. Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
  • Received:2007-08-19 Revised:2007-11-28 Online:2008-02-10 Published:2008-02-10
  • Contact: LIU Di

Abstract:

Adenosine monophosphate deaminase gene (AMPD1) plays an important role in the purine nucleotide cycle.The AMPD1 gene is expressed predominantly in skeletal muscles. It related with Inosine monophosphate metabolism in muscles and carcass traits. Herein the AMPD1 gene was cloned and a CDS sequence of 2 195 bp was obtained and analyzed with PCR-SSCP. χ2 analysis showed that the distribution of genotypes among Min pig, Yorkshire and Duroc are extremely sig-nificant different (P<0.01).