Abstract: SSCP (Single-strand conformation polymorphism, SSCP) technique was applied in this study to analyze the population genetic information about genetic distribution, variation and heterozygosity of PRKAB2(Protein kinase, AMP-activated, beta 2 non-catalytic subunit) gene in five purelines and three crossbreds. The genetic effects of PRKAB2 gene on fiber density and partial carcass traits were also analyzed. Only one point mutation T→C at base position 406 located among the first exon of the whole CDS was found in the whole CDS.The wild-type allele A had significant positive effects on live-weight, carcass-weight, leg-muscle-weight and abdomen-fat-weight (P<0.05), and had positive effects on meat tenderness.