Abstract:

As the most efficient strategy in gene clone, positional cloning has been used widely in QTL cloning in rice. The objective of this paper is to make summary features of QTL positional cloning based on 15 successful positional cloning attempts. (1) most of the populations used in the analysis were derived from interspecific or intersubspecies; (2) the target QTL had been identified with very large phenotypic effects; (3) the candidate genomic region was usually narrowed down to 40 kb; (4) the primary mapping result was exact; and the fine mapping population was more than 6 000, while the number of recessive population was more than 1 500. Otherwise, the nodus of QTL positional cloning and the corresponding solving methods were discussed.