利用改进的酚-氯仿法从猪毛囊中提取基因组DNA

doi:10.3724/SP.J.1005.2010.00752

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HEREDITAS ›› 2010, Vol. 32 ›› Issue (7): 752-756.doi: 10.3724/SP.J.1005.2010.00752

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Genomic DNA extraction from hair sacs of pigs using modified phe-nol-chloroform method

WANG Ji-Ying^{1, 2}, YU Ying¹, FENG Li-Xia¹, WANG Huai-Zhong², ZHANG Qin¹

1. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; 2. Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan 250100, China

Received:2009-12-28 Revised:2010-03-16 Online:2010-07-20 Published:2010-07-20
Contact: ZHANG Qin E-mail:3qzhang@cau.edu.cn

Abstract

Abstract: In referring to various methods for genomic DNA extraction from different tissues, we modified the classical phenol–chloroform procedure and reaction system for use in genomic DNA extraction from pig hair sacs. With the modified the phenol–chloroform method we successfully obtained high quality genomic DNA from pig hair sacs. Genomic DNA can be extracted from sacs of one to six pig hairs with satisfied quantity and quality for the need of PCR-based molecular ex-periment.

Key words: pig hair sac, genomic DNA extraction, phenol-chloroform method

WANG Ji-Yang, SHU Yang, FENG Li-Xia, WANG Fu-Zhong, ZHANG Qi. Genomic DNA extraction from hair sacs of pigs using modified phe-nol-chloroform method[J]. HEREDITAS, 2010, 32(7): 752-756.

Genomic DNA extraction from hair sacs of pigs using modified phe-nol-chloroform method

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