巴西橡胶树SSR遗传图谱的构建

doi:10.3724/SP.J.1005.2010.00857

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HEREDITAS ›› 2010, Vol. 32 ›› Issue (8): 857-863.doi: 10.3724/SP.J.1005.2010.00857

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Construction of genetic linkage map for rubber tree (Hevea brasiliensis)based on SSR markers

FENG Su-Ping^{1, 3}, LI Wei-Guo², YU Fei^{1, 4}, WANG Jing-Yi^{1, 4}, WU Yao-Ting³

1. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou 571101, China; 2. Rubber Cultivation Research Institute, Chinese Academy of Tropic Agricultural Science, Danzhou 571737, China; 3. Qiongzhou University, Wuzhishan 572200, China; 4. Agricultural College, Hainan University, Haikou 570228, China

Received:2009-10-16 Revised:2010-01-05 Online:2010-08-20 Published:2010-08-23
Contact: WU Yao-Ting E-mail:wuyaoting@tsinghua.org.cn

Abstract

Abstract: Out of 260 polymorphic loci screened from a total of 441 pairs of EST-SSR and genomic-SSR primers, 176 were used for constructing the genetic map of rubber tree (Hevea brasiliensis) by an F1 segregating population including 94 progenies from the cross Reyan 88-13×IAN873. Chi-square test carried out on the polymorphic loci used in constructing the map showed that 147 loci followed a segregation ratio of 1:1 and 12 loci followed a ratio of 1:2:1 and 17 loci followed a ratio of 1:1:1:1. Only 13 (7.38%) loci were distorted from the Mendelian ratio. The genetic linkage map consisted of 91 marker loci in 18 linkage groups and covered 1 937.06 cM with an average genetic distance of 21.29 cM between adjacent markers. The largest linkage group consisted of 16 marker loci, while the smallest one contained only 2 marker loci.

Key words: rubber tree (Hevea brasiliensis), SSR markers, genetic lingkage map

FENG Su-Ping, LI Wei-Guo, XU Fei, WANG Jing-Yi, WU Yao-Ting. Construction of genetic linkage map for rubber tree (Hevea brasiliensis)based on SSR markers[J]. HEREDITAS, 2010, 32(8): 857-863.

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Construction of genetic linkage map for rubber tree (Hevea brasiliensis)based on SSR markers

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