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HEREDITAS ›› 2005, Vol. 27 ›› Issue (3): 367-371.

• 研究报告 • Previous Articles     Next Articles

Generation of Chicken Germ-line Chimeras by Transferring PGCs and Their Identification by AFLP

HU Xiao-Fen1,3;XIE Bei1,4;YU Rui-Song;HUANG Qi-Zhong;ZHANG De-Fu;HUANG Lu-Sheng;LI Zhen   

  1. 1. Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai 201106,China ;2.Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences , Shanghai 201106,China;3.Jiangxi Provincial Key Laboratory for Animal Biotechnology,Jiangxi Agricultural University,Nanchang,330045,China;4.School of Life Science,Nanjing Normal University, Nanjing 210097,China
  • Received:2004-06-09 Revised:2004-09-14 Online:2005-06-10 Published:2005-06-10
  • Contact: LI Zhen

Abstract: PGCs (Primordial germ cells) were isolated from the blood of 51~56 h hatching Shiqiza chicken embryos by Ficoll density gradient centrifugation. The PGCs were injected into 2.5 d hatching embryos of H breed chicken to produce germ-line chimeras. AFLP checking method was established to identify chicken germline chimeras. Eight germ-line H-S chimera embryos were identified among 20 developing H breed embryos.