鸡L-FABP基因全长cDNA克隆表达及与杂种鸡脂肪沉积的关系

doi:10.3724/SP.J.1005.2011.00763

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HEREDITAS ›› 2011, Vol. 33 ›› Issue (7): 763-767.doi: 10.3724/SP.J.1005.2011.00763

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Whole cDNA sequence cloning and expression of chicken L-FABP gene and its relationship with lipid deposition of hybrid chickens

YU Ying¹, WANG Dong^1,2, SUN Dong-Xiao¹, XU Gui-Yun¹, LI Jun-Ying¹, ZHANG Yuan¹

1. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China 2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2010-09-27 Revised:2011-03-02 Online:2011-07-20 Published:2011-07-25

Abstract

Abstract: Liver fatty acid-binding protein (L-FABP) is closely related to intracellular transportation and deposition of lipids. A positive differential displayed fragment was found in the liver tissue among Silkie (CC), CAU-brown chicken (CD), and their reciprocal hybrids (CD and DC) at 8 weeks-old using differential display RT-PCR techniques (DDRT-PCR). Through recycling, sequencing, and alignment analysis, the fragment was identified as chicken liver fatty acid-binding protein gene (L-FABP, GenBank accession number AY321365). Reverse Northern dot blot and semi-quantitative RT-PCR re-vealed that the avian L-FABP gene was over-expressed in the liver tissue of the reciprocal hybrids (CD and DC) compared to their parental lines (CC and DD), which was consistent with the fact that higher abdomen fat weight and wider inter-muscular fat width observed in the reciprocal hybrids. Considering the higher expression of L-FABP may contribute to the increased lipid deposition in the hybrid chickens, the functional study of avian L-FABP is warranted in future.

Key words: L-FABP, fatty trait, Silkie, CAU-brown chicken, reciprocal hybrids

SHU Yang, WANG Dong, SUN Dong-Xiao, XU Gui-Yun, LI Dun-Yang, ZHANG Yuan. Whole cDNA sequence cloning and expression of chicken L-FABP gene and its relationship with lipid deposition of hybrid chickens[J]. HEREDITAS, 2011, 33(7): 763-767.

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Whole cDNA sequence cloning and expression of chicken L-FABP gene and its relationship with lipid deposition of hybrid chickens

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