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HEREDITAS ›› 2005, Vol. 27 ›› Issue (3): 377-381.

• 研究报告 • Previous Articles     Next Articles

RAPD Identification of Three Types of Three-lines Hybrid Rice with Different Male Sterile Cytoplasms and Their Parent Lines

XU Xin 1,2;LIU Xue-Qun1,2;QU Bo 3;ZHU Ying-Guo2;WANG Chun-Tai1   

  1. 1. Biotechnological Laboratory of National Commission for Nationalities, College of Chemistry and Life Science, South–central University for Nationalities, Wuhan 430074,China; 2. Key Laboratory of MOE for Plant Developmental Biology, College of Life Science, Wuhan University, Wuhan 430072 China; 3.College of Plant Science and Technology, Huazhong Agricultural University,Wuhan 430074 China
  • Received:2004-06-14 Revised:2004-12-15 Online:2005-06-10 Published:2005-06-10
  • Contact: WANG Chun-Tai

Abstract: The RAPD analysis was conducted on genome DNA from 21 sets of rice, including 6 three–line hybrid rice combinations, separately derived from three different kinds of cytoplasmic male sterile (CMS) lines and their related parents. Out of 264 random primers screened first, 25 primers displayed well in polymorphisms. It was shown that only 7 bands amplified respectively from 7 primers were enough to discriminate the different types of CMS, the hybrid combinations and their parents.

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