Cre/lox位点特异性重组系统在高等真核生物中的研究进展

doi:10.3724/SP.J.1005.2012.00177

[an error occurred while processing this directive]

HEREDITAS ›› 2012, Vol. 34 ›› Issue (2): 177-189.doi: 10.3724/SP.J.1005.2012.00177

• en • Previous Articles Next Articles

Progress in Cre/lox site-specific recombination system in higher eukaryotes

LONG Ding-Pei¹, TAN Bing^1,2, ZHAO Ai-Chun¹, XU Long-Xia^1,3, XIANG Zhong-Huai¹

1. Institute of Sericulture and Systems Biology, Key Sericultural Laboratory of the Ministry of Agriculture, Southwest University, Chongqing 400716, China 2. Molecular Virology and Viral Immunology Research Group, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China 3. Shanghai Institute of Biological Sciences, Institute of Biochemistry and Cell Biology, Shanghai 200031, China

Received:2011-06-03 Revised:2011-09-07 Online:2012-02-20 Published:2012-02-25

Abstract

Abstract: Cre/lox system derived from P1 bacteriaphage can quickly and effectively achieve gene insertion, deletion, replacement, and inversion by means of site-specific recombination. As one of the most important tools for gene targeting at present, Cre/lox system has been widely used in Arabidopsis thaliana, Oryza sativa L., Mus musculus, Drosophila melanogaster, Danio rerio, and other higher eukaryotic organisms. This review roundly described the basic profile of Cre/lox system, and its application in higher eukaryotes. In addition, we also discussed the main problems and developmental trend of the Cre/lox system in this review, which can be a good reference for using Cre/lox system to realize the gene manipulations of the different high eukaryotic organisms.

Key words: Cre/lox recombination system, gene manipulation, site-specific recombination, gene targeting

LONG Ding-Pei, TAN Bing, ZHAO Ai-Chun, XU Long-Xia, XIANG Zhong-Huai. Progress in Cre/lox site-specific recombination system in higher eukaryotes[J]. HEREDITAS, 2012, 34(2): 177-189.

References

[1] 滕艳, 杨晓. 基因打靶技术: 开启遗传学新纪元. 遗传, 2007, 29(11): 1291-1298.
[2] 谷欣, 黎燕. 位点特异性重组技术研究进展. 生物技术通讯, 2005, 16(4): 417-419.
[3] Landy A. Coming or going it’s another pretty picture for the λ-Int family album. Proc Natl Acad Sci USA, 1999, 96(13): 7122-7124.
[4] Nagy A. Cre recombinase: the universal reagent for genome tailoring. Genesis, 2000, 26(2): 99-109.
[5] Sternberg N, Hamilton D. Bacteriophage P1 site-specific recombination. I. Recombination between loxP sites. J Mol Biol, 1981, 150(4): 467-486.
[6] Abremski K, Hoess R, Sternberg N. Studies on the proper-ties of P1 site-specific recombination: evidence for topo-logically unlinked products following recombination. Cell, 1983, 32(4): 1301-1311.
[7] Sternberg N. Demonstration and analysis of P1 site-specific recombination using λ-P1 hybrid phages con-structed in vitro. Cold Spring Harb Symp Quant Biol, 1979, 43(Pt 2): 1143-1146.
[8] Sternberg N, Sauer B, Hoess R, Abremski K. Bacterio-phage P1 cre gene and its regulatory region. Evi-dence for multiple promoters and for regulation by DNA methylation. J Mol Biol, 1986, 187(2): 197-212.
[9] Guo F, Gopaul DN, van Duyne GD. Structure of Cre recombinase complexed with DNA in a site-specific recom-bination synapse. Nature, 1997, 389(6646): 40-46.
[10] van Duyne GD. A structural view of Cre-loxP site-specific recombination. Annu Rev Biophys Biomol Struct, 2001, 30: 87-104.
[11] Kim ST, Kim GW, Lee YS, Park JS. Characterization of Cre-loxP interaction in the major groove: hint for structural distortion of mutant Cre and possible strategy for HIV-1 therapy. J Cell Biochem, 2001, 80(3): 321-327.
[12] Hartung M, Kisters-Woike B. Cre mutants with altered DNA binding properties. J Biol Chem, 1998, 273(36): 22884-22891.
[13] Hoess RH, Ziese M, Sternberg N. P1 site-specific recom-bination: nucleotide sequence of the recombining sites. Proc Natl Acad Sci USA, 1982, 79(11): 3398-3402.
[14] Hoess RH, Abremski K. Interaction of the bacteriophage P1 recombinase Cre with the recombining site loxP. Proc Natl Acad Sci USA, 1984, 81(4): 1026-1029.
[15] Abremski K, Hoess R, Sternberg N. Studies on the properties of P1 site-specific recombination: evidence for topo-logically unlinked products following recombination. Cell, 1983, 32(4): 1301-1311.
[16] Hoess RH, Wierzbicki A, Abremski K. The role of the loxP spacer region in PI site-specific recombina-tion. Nucl Acids Res, 1986, 14(5): 2287-2300.
[17] Abremski K, Hoess R. Phage P1 Cre-loxP site-specific recombination: Effects of DNA supercoiling on catenation and knotting of recombinant products. J Mol Biol, 1985, 184(2): 211-220.
[18] Albert H, Dale EC, Lee E, Ow DW. Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome. Plant J, 1995, 7(4): 649-659.
[19] Thomson JG, Rucker EB III, Piedrahita JA. Mutational analysis of LoxP sites for efficient Cre-mediated insertion into genomic DNA. Genesis, 2003, 36(3): 162-167.
[20] Araki K, Okada Y, Araki M, Yamamura K. Comparative analysis of right element mutant lox sites on recombination efficiency in embryonic stem cells. BMC Bio-technol, 2010, 10: 29.
[21] Mack A, Sauer B, Abremski K, Hoess R. Stoichiometry of the Cre recombinase bound to the lox recombining site. Nucl Acids Res, 1992, 20(17): 4451-4455.
[22] Hoess RH, Abremski K. Mechanism of strand cleavage and exchange in the Cre-lox site-specific recom-bination system. J Mol Biol, 1985, 181(3): 351-362.
[23] Martin SS, Pulido E, Chu VC, Lechner TS, Baldwin EP. The order of strand exchanges in Cre-LoxP recombination and its basis suggested by the crystal structure of a Cre-LoxP Holliday junction complex. J Mol Biol, 20

Metrics

Comments

www.chinagene.cn
备案号：京ICP备09063187号

Progress in Cre/lox site-specific recombination system in higher eukaryotes

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 11

Recommended Articles

Metrics

Comments

[1]	SHEN Yan, HUANG Peng, ZHANG Bo. A protocol for TALEN construction and gene targeting in zebrafish [J]. HEREDITAS, 2013, 35(4): 533-544.
[2]	XIAO An, HU Ying-Ying, WANG Wei-Ye, YANG Zhi-Peng, WANG Zhan-Xiang, HUANG Feng, TONG Xiang-Jun, ZHANG Bo, LIN Shuo. Progress in zinc finger nuclease engineering for targeted genome modification [J]. HEREDITAS, 2011, 33(7): 665-683.
[3]	LUO Qiang-Miao, MIAO Xiang-Yang, ZHANG Rui-Jie. An update on the development of transgenic animal technology [J]. HEREDITAS, 2011, 33(5): 449-458.
[4]	LIN Fu-Yu, YANG Xiao. Issues and solutions of conditional gene targeting [J]. HEREDITAS, 2011, 33(5): 469-484.
[5]	ZHONG Jiang, DIAO Shu-Gong. The mechanism and application of zinc finger nucleases [J]. HEREDITAS, 2011, 33(2): 123-130.
[6]	SUN Zhen-Gong, MIAO Xiang-Yang, SHU Rui-Liang. New advances in animal transgenic technology [J]. HEREDITAS, 2010, 32(6): 539-547.
[7]	XU Huan-Yu, GONG Xiu-Li, GUO Xin-Bing, MA Qing-Wen, ZENG Yi-Tao. Construction of the oocyte-specific expressing fC31 integrase vector pZP3-INT and its expression in mouse oocytes [J]. HEREDITAS, 2009, 31(6): 595-599.
[8]	ZHOU Yin, WANG Yue-Ju, WANG Ying. Application prospect of new site-specific recombination systems in gene transformation and gene stacking [J]. HEREDITAS, 2008, 30(2): 149-154.
[9]	TENG Yan, YANG Xiao. Gene targeting: the beginning of a new era in genetics [J]. HEREDITAS, 2007, 29(11): 1291-1298.
[10]	YIN Xiu-Shan, ZHANG Ling-Qiang, HE Fu-Chu. The Application of RNAi Technology in Transgenic Mice [J]. HEREDITAS, 2006, 28(3): 351-356.
[11]	LI Xiang-Ping, XU Wei-Zhuo, LI Ning. Research Progress and Prospect of Animal Gene Targeting [J]. HEREDITAS, 2003, 25(1): 81-88.