基于线粒体和核基因序列的蜜蜂属系统发育分析

doi:10.3724/SP.J.1005.2012.01057

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HEREDITAS ›› 2012, Vol. 34 ›› Issue (8): 1057-1063.doi: 10.3724/SP.J.1005.2012.01057

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Phylogenetic relationships of honey bees (Hymenoptera: Apinae) in-ferred from mitochondrial and nuclear DNA sequences

CAO Lian-Fei¹, NIU De-Fang¹, HE Shao-Yu², KUANG Hai-Ou², HU Fu-Liang¹

1. College of Animal Sciences, Zhejiang University, Hangzhou 310058, China 2. Eastern Bee Research Institute, Yunnan Agricultural University, Kunming 650201, China

Received:2012-01-06 Revised:2012-02-24 Online:2012-08-20 Published:2012-08-25

Abstract

Abstract: The sequences of mitochondrial ND2, CO2, and 16S rRNA genes and nuclear ITPR gene were obtained from 22 samples of 5 Apis species from China. The characteristics of the sequences and the pairwise distances among species were analyzed. Phylogenetic trees were reconstructed for Apis species using maximum parsimony, neighbor-joining and maximum likelihood methods together with the sequences of the other 4 Apis species downloaded from GenBank. Results supported that Apis species were divided into three major clusters: dwarf bees (A. florea and A. andreniformis), giant bees (A. dorsata and A. laboriosa), and cavity-nesting bees (A. mellifera, A. cerana, A. koschevnikovi, A. nigrocinta, and A. nuluensis). The dwarf honey bees were confirmed as basal lineage. Our study also revealed a high level of genetic divergence between A. dorsata from Hainan Island and China mainland.

Key words: Apis, ND2, CO2, 16S rRNA, ITPR, molecular phylogeny

CAO Lian-Fei, NIU De-Fang, HE Shao-Yu, KUANG Hai-Ou, HU Fu-Liang. Phylogenetic relationships of honey bees (Hymenoptera: Apinae) in-ferred from mitochondrial and nuclear DNA sequences[J]. HEREDITAS, 2012, 34(8): 1057-1063.

References

[1] Morse RA, Calderone NW. The value of honey bees as pollinators of U.S. crops in 2000. Bee Culture, 2000, 128: 1-15.
[2] 郑火青, 胡福良. 蜜蜂－新兴的模式生物. 昆虫学报, 2009, 52(2): 210-215.
[3] Oldroyd BP, Wongsiri S. Asian Honey Bees: Biology, Conservation, and Human Interactions. Cambridge: Harvard University Press, 2006: 13-35.
[4] Koeniger N, Koeniger G, Smith D. Phylogeny of the Genus Apis. In: Hepburn HR, Radloff SE, eds. Hon-eybees of Asia. Berlin: Springer, 2011: 23-50.
[5] Arias MC, Sheppard WS. Phylogenetic relationships of honey bees (Hymenoptera: Apinae: Apini) inferred from nuclear and mitochondrial DNA sequence data. Mol Phylogenet Evol, 2005, 37(1): 25-35.
[6] Raffiudin R, Crozier RH. Phylogenetic analysis of honey bee behavioral evolution. Mol Phylogenet Evol, 2007, 43(2): 543-552.
[7] Lo N, Gloag RS, Anderson DL, Oldroyd BP. A molecular phylogeny of the genus Apis suggests that the Gi-ant Honey Bee of the Philippines, A. breviligula Maa, and the Plains Honey Bee of southern India, A. indica Fabricius, are valid species. Syst Ento-mol, 2010, 35(2): 226- 233.
[8] 吴燕如. 中国动物志昆虫纲, 第二十卷. 膜翅目: 准蜂科-蜜蜂科. 北京: 科学出版社, 2000: 370-383.
[9] Hepburn HR, Radloff SE. Biogeography. In: Hepburn HR, Radloff SE, eds. Honeybees of Asia. Berlin: Springer, 2011: 62-63.
[10] Crozier RH, Crozier YC. The mitochondrial genome of the honeybee Apis mellifera: complete sequence and genome organization. Genetics, 1993, 133(1): 97-117.
[11] Willis LG, Winston ML, Honda BM. Phylogenetic relationships in the honeybee (genus Apis) as determined by the sequence of the cytochrome oxidase II region of mitochondrial DNA. Mol Phylogenet Evol, 1992, 1(3): 169-178.
[12] Cameron SA, Derr JN, Austin AD, Wharton RA. The application of nucleotide sequence data to phylogeny of the Hymenoptera: a review. J Hym Res, 1992, 1(1): 63-79.
[13] Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res, 1997, 25(24): 4876-4882.
[14] Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol, 2007, 24(8): 1596-1599.
[15] Swofford DL. PAUP*: Phylogenetic analysis using parsimony (and other methods), Version 4. Sunderland, MA: Sinauer Associates, 2003.
[16] Posada D, Crandall KA. MODELTEST: testing the model of DNA substitution. Bioinformatics, 1998, 14(9): 817-818.

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Phylogenetic relationships of honey bees (Hymenoptera: Apinae) in-ferred from mitochondrial and nuclear DNA sequences

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