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HEREDITAS ›› 2012, Vol. 34 ›› Issue (9): 1174-1180.doi: 10.3724/SP.J.1005.2012.01174

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Analysis of expression pattern of zebrafish leg1 homologus gene mu-leg1 in mouse

ZHU Zhi-Hui, HU Min-Jie, CHANG Chang-Qing, PENG Jin-Rong   

  1. College of Animal Sciences, ZhejiangUniversity, Hangzhou 310058, China
  • Received:2012-03-13 Revised:2012-04-06 Online:2012-09-20 Published:2012-09-25

Abstract: Gene leg1 (liver-enriched gene 1) was first identified as a novel gene whose expression was enriched in the liver of zebrafish. Further studies revealed that Leg1 protein was a novel secretory protein, which played a role in the liver development in zebrafish. Here we reported the analysis of expression pattern of zb-leg1 homologus gene mu-leg1. The cDNA of mu-leg1 was isolated from adult mouse liver by nested PCR. This gene encodes a putative protein, mu-Leg1, which shares 31% similarity with zb-Leg1 of zebrafish. Both Northern blotting and semi-quantitative RT-PCR demonstrated that the expression of mu-leg1 was enriched in the small intestine rather than in the liver in adult mouse. We also produced a recombined mu-Leg1 protein and a mu-Leg1 specific antibody. West-ern blottingdemonstrated that mu-Leg1 was a secretory protein. In addition, we have established a mu-leg1 condi-tional knock-out heterozygous mouse. Our work builds a basis for further studies of mu-leg1.

Key words: mu-leg1, recombinant protein, polyclonal antibody, conditional knock-out mouse