酵母双杂交筛选与GsCBRLK相互作用的蛋白质

doi:10.3724/SP.J.1005.2013.00388

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HEREDITAS ›› 2013, Vol. 35 ›› Issue (3): 388-394.doi: 10.3724/SP.J.1005.2013.00388

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Identification of calcium/calmodulin-binding receptor-like kinase GsCBRLK-interactive proteins using yeast two-hybrid system

YANG Shan-Shan, SUN Xiao-Li, YU Yang, CAI Hu, JI Wei, BAI Xi, ZHU Yan-Ming

Laboratory of Plant Bioengineering, College of Life Sciences, Northeast Agriculture University, Harbin 150030, China

Received:2012-09-11 Revised:2013-01-25 Online:2013-03-20 Published:2013-03-25

Abstract

Abstract: GsCBRLK (calcium/calmodulin-binding receptor-like kinase from Glycine soja) links ABA (abscisic acid)- and salt-induced calcium/calmodulin signal in plant cells. In order to study the molecular mechanismes of GsCBLRK, the salt-treated Glycine soja cDNA library was screened with pBT3-STE-CBRLK as bait plasmid using yeast two hybrid system. Two positive clones (SNARE and 14-3-3 protein) were identified by constructing cDNA library of wild soybean under salt treatment, membrane system yeast two hybrid screening, multiple screen, rotary validation, bioinformatic analysis and in-teraction identification in yeast.

Key words: GsCBRLK, yeast two-hybrid system, protein interaction, bioinformatic analysis

CLC Number:

Q789

YANG Shan-Shan SUN Xiao-Li YU Yang CAI Hu JI Wei BAI Xi ZHU Yan-Ming. Identification of calcium/calmodulin-binding receptor-like kinase GsCBRLK-interactive proteins using yeast two-hybrid system[J]. HEREDITAS, 2013, 35(3): 388-394.

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Identification of calcium/calmodulin-binding receptor-like kinase GsCBRLK-interactive proteins using yeast two-hybrid system

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