对家蚕第18连锁群隐性基因elp、ch-2和mln测交系的分子定位分析

doi:10.3724/SP.J.1005.2013.00373

[an error occurred while processing this directive]

HEREDITAS ›› 2013, Vol. 35 ›› Issue (3): 373-378.doi: 10.3724/SP.J.1005.2013.00373

• en • Previous Articles Next Articles

Molecular mapping of test mapping strain for 18th linkage group re-cessive genes elp, ch-2 and mln in silkworm (Bombyx mori)

LIU Xian-Fang¹, MA Xiao³, HOU Cheng-Xiang², LI Bing¹, LI Mu-Wang^1,2

1. Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, China 2. Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China 3. College of Biotechnology and Environmental Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, China

Received:2012-10-11 Revised:2012-11-21 Online:2013-03-20 Published:2013-03-25

Abstract

Abstract: The ellipsoid egg, the second recessive gene of chocolate larvae, and melanism are controlled by three recessive genes, elp, ch-2, and mln in silkworm, respectively. Their order and genetic distance have been scheduled in established linkage group. Owing to lack of crossing over in females, the reciprocal backcrossed F1(BC1) progenies were bred for linkage analysis using the wild type silkworm strain P50(+elp+ch-2+mln /+elp+ch-2+mln) and W18 with ellipsoid egg, the second recessive gene of chocolate larvae, and melanism (elp ch-2 mln / elp ch-2 mln). In this research, we mapped three mutant genes, elp, ch-2, and mln on the chromosome 18 based on the SSR linkage map and STS markers designed based on silkworm genome sequence. The established linkage group, molecular linkage group, and the physic map of chromosome 18 had been corresponded. The genetic distance for this chromosome in this research was 94.2 cM, and the order of the mutants and molecular markers were consistent with the established silkworm linkage maps and the fine ge-nome sequences. This research will lay important bases for map-based cloning for other mutants on chromosome 18.

Key words: Bombyx mori, 18th chromosome, mutant, molecular marker, gene mapping

LIU Xian-Fang MA Xiao HOU Cheng-Xiang LI Bing LI Mu-Wang. Molecular mapping of test mapping strain for 18th linkage group re-cessive genes elp, ch-2 and mln in silkworm (Bombyx mori)[J]. HEREDITAS, 2013, 35(3): 373-378.

References

[1] 吉武成美, 蒋猷龙. 家蚕的起源和分化. 蚕业科学, 1987, 13(3): 23-25.
[2] 秦俭. 家蚕第二隐性赤蚁的遗传学研究初报. 蚕业科学, 1985, 11(2): 103-106.
[3] 鲁成, 向仲怀. 家蚕新突变型淡赤蚁的遗传学研究. 蚕业科学, 1990, 16(1): 21-24.
[4] Fujii H, Banno Y, Doira H, Kawaguchi Y. Genetical stocks and mutations of Bombyx mori: important genetic resources, 2nd edn. Fukuoka: Institute of Genetic Re-sources, Faculty of Agriculture, Kyusyu University, 1998.
[5] Kyeth WS. Studies on the inheritance of cocoon color and egg shape in Bombyx mori. The doctoral thesis. Fukuoka: Kyushu University, 1943: 182.
[6] 鲁成, 向仲怀, 陈家莲. 家蚕长形卵(elp)卵壳的特异构造. 蚕学通讯, 1991, 11(1): 17-19.
[7] Kan H, Kim CH, Kwon HM, Park JW, Roh KB, Lee H, Park BJ, Zhang R, Zhang JH, Söderhäll K, Ha NC, Lee BL. Molecular control of phenoloxidase-induced melanin syn-thesis in an insect. J Biol Chem, 2008, 283(37): 25316-25323.
[8] 代方银, 谭端, 童晓玲, 胡海, 鲁成, 向仲怀. 含有母性影响遗传基因的连锁定位系的构建. 遗传, 2007, 29(11): 1393-1398.
[9] Yasukochi Y. A dense genetic map of the silkworm, Bombyx mori, covering all chromosomes based on 1018 molecular markers. Genetics, 1998, 150(4): 1513-1525.
[10] Shi JR, Heckel DG, Goldsmith MR. A genetic linkage map for the domesticated silkworm, Bombyx mori, based on restriction fragment length polymorphisms. Genet Res, 1995, 66(2): 109-126.
[11] Tan YD, Wan CL, Zhu YF, Lu C, Xiang ZH, Deng HW. An amplified fragment length polymorphism map of the silkworm. Genetics, 2001, 157(3): 1277-1284.
[12] Promboon A, Shimada T, Fujiwara H, Kobayashi M. Linkage map of random amplified polymorphic DNAs (RAPDS) in the silkworm, Bombyx mori. Genet Res, 1995, 66(1): 1-7.
[13] Miao XX, Xu SJ, Li MH, Li MW, Huang JH, Dai FY, Marino SW, Mills DR, Zeng PY, Mita K, Jia SH, Zhang Y, Liu WB, Xiang H, Guo QH, Xu AY, Kong XY, Lin HX, Shi YZ, Lu G, Zhang XL, Huang W, Yasukochi Y, Su-gasaki T, Shimada T, Nagaraju J, Xiang ZH, Wang SY, Goldsmith MR, Lu C, Zhao GP, Huang YP. Simple se-quence repeat-based consensus linkage map of Bombyx mori. Proc Natl Acad Sci USA, 2005, 102(45): 16303-16308.
[14] Yamamoto K, Narukawa J, Kadono-Okuda K, Nohata J, Sasanuma M, Suetsugu Y, Banno Y, Fujii H, Goldsmith MR, Mita K. Construction of a single nucleotide poly-morphism linkage map for the silkworm, Bombyx mori, based on bacterial artificial chromosome end sequences. Genetics, 2006, 173(1): 151-161.
[15] Olson M, Hood L, Cantor C, Botstein D. A common lan-guage for physical mapping of the human genome. Science, 1989, 245(4925): 1434-1435.
[16] Chandra A, Kaushal P. Identification of diploid Stylosan-thes seabrana accessions from existing germplasm of S. scabra utilizing genome-specific STS markers and flow cytometry, and their molecular characterization. Mol Biotechnol, 2009, 42(3): 282-291.
[17] Tautz D. Hypervariability of simple sequences as a gen-eral source for polymorphic DNA markers. Nucleic Acids Res, 1989, 17(16): 6463-6471.
[18] 侯成香, 李木旺, 张月华, 钱荷英, 孙平江, 徐安英, 苗雪霞, 郭秋红, 相辉, 黄勇平. 利用SSR标记进行家蚕部分品种资源的指纹图谱分析. 中国农业科学, 2006, 39(10): 2124-2131.
[19] The International Silkworm Genome Consortium. The genome of a lepidopteran model insect, the silkworm Bombyx mori. Insect Biochem Mol Biol, 2008, 38(12): 1036-1045.
[20] Xiang H, Li M, Yang F, Guo Q, Zhan S, Lin H, Miao X, Huang Y. Fine mapping of EKp-1, a locus associated with silkworm (Bombyx mori) proleg development. He-redity, 2008, 100(5): 533-540.
[21] Ito K, Kidokoro K, Sezutsu H, Nohata J, Yamamoto K, Kobayashi I, Uchino K, Kalyebi A, Eguchi R, Hara W, Tamura T, Katsuma S, Shimada T, Mita K, Kadono-Okuda K. Deletion of a gene encoding an amino acid transporter in the midgut membrane causes resistance to a Bombyx parvo-like virus. Proc Natl Acad Sci USA, 2008, 105(21): 7523-7527.
[22] 王修业, 李木旺, 赵云坡, 徐安英, 郭秋红, 黄勇平, 郭锡杰. 结合SSR标记和STS标记对家蚕无鳞毛翅基因的定位. 遗传, 2010, 31(1): 54-58.
[23] 张蕊, 梅兴林, 王修业, 郭秋红, 李冰, 汪生鹏, 徐安英, 黄勇平, 郭锡杰, 李木

Metrics

Comments

www.chinagene.cn
备案号：京ICP备09063187号

Molecular mapping of test mapping strain for 18th linkage group re-cessive genes elp, ch-2 and mln in silkworm (Bombyx mori)

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Kailun Li, Jingao Lu, Xiaohui Chen, Wenqing Zhang, Wei Liu. The role of the allantoin in promoting fracture healing in osteoclast-deficient zebrafish [J]. Hereditas(Beijing), 2023, 45(4): 341-353.
[2]	Xueying Huang,Kai Fan,Yanfang Ye,Bin Wang,Weiren Wu,Tao Lan. Teaching practice and experiences of verifying the three laws of genetics based on the SSLP marker analysis [J]. Hereditas(Beijing), 2017, 39(9): 856-862.
[3]	Xiaobin Han, Ran Xu, Penggen Duan, Haiyue Yu, Yuehua Luo, Yunhai Li, . Genetic analysis and identification of candidate genes for two spotted-leaf mutants (spl101 and spl102) in rice [J]. Hereditas(Beijing), 2017, 39(4): 346-353.
[4]	Jinhong Yuan, Junhua Li, Jiaojiao Yuan, Keli Jia, Shufen Li, Chuanliang Deng, Wujun Gao. The application of MutMap in forward genetic studies based on whole-genome sequencing [J]. Hereditas(Beijing), 2017, 39(12): 1168-1177.
[5]	Haibing Song, Bin Wang, Renjie Chen, Xiaoya Zheng, Shibo Yu, Tao Lan. Genetic analysis and gene mapping of the glabrous leaf and hull mutant glr3 in rice (Oryza sativa L.) [J]. Hereditas(Beijing), 2016, 38(11): 1012-1019.
[6]	Haifeng Li, Huiping Liu, Yi Dai, Shuai Huang, Jun Zhang, Yong Gao, Jianmin Chen. Transmitting characters of individual E chromosomes of Thinopyrum elongatum in Triticum turgidum background [J]. Hereditas(Beijing), 2016, 38(11): 1020-1029.
[7]	Yungao Hu, Lianan Guo, Guotao Yang, Peng Qin, Cunliu Fan, Youlin Peng, Wei Yan, Hang He, Shigui Li. Genetic analysis of dense and erect panicle 2 allele DEP2-1388 and its application in hybrid rice breeding [J]. HEREDITAS(Beijing), 2016, 38(1): 72-81.
[8]	Cairui Lu, Changsong Zou, Guoli Song. Recent progress in gene mapping through high-throughput sequencing technology and forward genetic approaches [J]. HEREDITAS(Beijing), 2015, 37(8): 765-776.
[9]	Xueqian Li, Ran Xu, Penggen Duan, Yingbao Wu, Yuehua Luo, Yunhai Li. Genetic analysis and identification of candidate genes for a narrow leaf mutant (zy17) in rice [J]. HEREDITAS(Beijing), 2015, 37(6): 582-589.
[10]	Dan Zhang,Haina Song,Hao Cheng,Deyue Yu. Mapping and cloning of low phosphorus tolerance genes in soybeans [J]. HEREDITAS(Beijing), 2015, 37(4): 336-343.
[11]	Hui Wang， Guang Li， Yiquan Wang. Generating amphioxus Hedgehog knockout mutants and phenotype analysis [J]. HEREDITAS(Beijing), 2015, 37(10): 1036-1043.
[12]	Min Bai， Qi Li， Yanjiao Shao， Yuanhua Huang， Dali Li， Yanlin Ma. Generation of site-specific mutant mice using the CRISPR/Cas9 system [J]. HEREDITAS(Beijing), 2015, 37(10): 1029-1035.
[13]	Fen Li, Jinghui Yang, Xiuli Wu, Weilin Zhang, Guanfeng Wang, Xiaojie Yue. Effects of histone H3 K4L and K36L mutations on the cell growth and the transcription of GAL1, SSA3 and PHO5 in Saccharomyces cerevisiae [J]. HEREDITAS(Beijing), 2014, 36(8): 827-834.
[14]	Qiaoling Zhao, Wenbo Wang, Anli Chen, Zhiyong Qiu, Dingguo Xia, Heying Qian, Xingjia Shen. Genetic analysis and gene mapping of two novel quail-like mutants from the silkworm (Bombyx mori) [J]. HEREDITAS, 2014, 36(4): 369-375.
[15]	Shaojuan Song, Yaping Guo, Xueyao Zhang, Jianzhen Zhang, Enbo Ma. Screening and SNP mapping of copper-resistant mutations in C. elegans [J]. HEREDITAS(Beijing), 2014, 36(12): 1261-1268.