四倍体小麦背景中长穗偃麦草E染色体传递特征

doi:10.16288/j.yczz.16-107

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Hereditas(Beijing) ›› 2016, Vol. 38 ›› Issue (11): 1020-1029.doi: 10.16288/j.yczz.16-107

• Research Articles • Previous Articles Next Articles

Transmitting characters of individual E chromosomes of Thinopyrum elongatum in Triticum turgidum background

Haifeng Li^{1, 2}, Huiping Liu¹, Yi Dai¹, Shuai Huang³, Jun Zhang², Yong Gao¹, Jianmin Chen¹

1. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China;
2. Yangzhou Polytechnic College, Yangzhou 225012, China;
3. Jiangsu Provincial Key Lab for Agrobiology, Nanjing 210014, China

Received:2016-03-29 Online:2016-11-20 Published:2016-06-29
Supported by:
[Supported by the National Natural Science Foundation of China(No. 31071406), the Specialized Research Fund for Doctoral Program of Higher Education of China (No. 20123250110010) and the Research Fund of Jiangsu Provincial Key Lab for Agrobiology]

Abstract

Abstract: The transmission patterns of Thinopyrum elongatum chromosomes in the background of Triticum turgidum were investigated through cytogenetic and molecular marker analysis based on the F₂ and F₃ plants derived from a cross between Triticum trititrigia (AABBEE) and T. turgidum L. ssp. durum (AABB). An additional objective was to develop durum-Th. elongatum E chromosome addition lines. Among 218 F₂plants, individuals with 2n=28 accounted for 41.7%, those with 2n=29 accounted for 18.3%, and the remaining 40.0% had 2n=31-42. Molecular marker analysis of the F₂ monosomic addition plants with 2n=29 showed significant differences in transmission rate among Th. elongatum chromosomes. Chromosome 1E had the highest transmission rates, while 3E and 6E had the lowest. Among F₂ double monosomic addition plants with 2n=30, joint transmission was frequent between 1E, 4E, 7E and 5E; 6E was less frequent in joint transmission with other chromosomes, while 2E and 3E never transmitted along with other chromosomes. Among F₃ plants derived from F₂ monosomic addition plants, the transmission rates of E chromosomes varied widely, from the minimum of 9.1% for 6E to the maximum of 27.5% for 1E, consistent with observations from F₂ plants. A full set of durum-Th. elongatum 1E-7E monosomic addition lines and several disomic addition lines were selected from F₃ plants, and all monosomic addition plants were fertile. These chromosome E addition lines will be useful intermediate materials for developing substitution lines and translocation lines.

Key words: Triricum trititrigia (6×), Triticum turgidum, Thinopyrum elongatum, transmission rate, chromosome-specific molecular markers

Haifeng Li, Huiping Liu, Yi Dai, Shuai Huang, Jun Zhang, Yong Gao, Jianmin Chen. Transmitting characters of individual E chromosomes of Thinopyrum elongatum in Triticum turgidum background[J]. Hereditas(Beijing), 2016, 38(11): 1020-1029.

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Transmitting characters of individual E chromosomes of Thinopyrum elongatum in Triticum turgidum background

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