纳米银颗粒及纳米二氧化钛颗粒的体外遗传毒性研究

doi:10.16288/j.yczz.20-161

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Hereditas(Beijing) ›› 2020, Vol. 42 ›› Issue (12): 1192-1200.doi: 10.16288/j.yczz.20-161

• Research Article • Previous Articles Next Articles

In vitro genotoxicity study of silver nanoparticles and titanium dioxide nanoparticles

Haimei Zhu¹, Pengcheng Huang², Tiantian Zhao³, Changhui Zhou^2,³, Ruowan Li³, Chunrong Yu^2,³, Zhiyong Chen², Linfeng Gu², Yan Chang^1,^2,³()

^1. School of Chemistry and Chemical Engineer, Shanghai University of Engineering Science, Shanghai 201620, China
^2. Shanghai Innostar Bio-tech Co. Ltd, Shanghai 201203, China
^3. China State Institute of Pharmaceutical Industry, Shanghai 201201, China

Received:2020-07-18 Revised:2020-10-08 Online:2020-12-17 Published:2020-11-09
Contact: Chang Yan E-mail:ychang@ncdser.com
Supported by:
Supported by “the13th Five-Year plan” Major New Drug Innovation and Technology Major Project No(2018ZX09201017-008);the Shanghai Municipal Science and Technology Commission R&d Public Service Platform Project No(18DZ2290100)

Abstract

Abstract:

Nanoparticles are widely used in cosmetic, pharmaceutical, and food industries, but their safety and genetic toxicity are still unclear. In this study, the genotoxicity of silver nanoparticles (AgNPs) and titanium dioxide nanoparticles (titanium dioxide nanoparticles) were evaluated by in vitro comet assay and PIG-A assay in TK6 cells. We exposed TK6 cells to two types of nanoparticles at the highest concentration of 200 μmol/L for 4 h and conducted the in vitro comet assay. We examined the mutation results of PIG-A gene in vitro after 4 h, 24 ho and 10 days of exposure, respectively. We also examined the endocytosis of nanoparticles in TK6 cells exposed to nanoparticles for 24 h. In the endocytosis assay, with the increase of nano-material concentration, the side scatter (SSC) of TK6 cells in flow cytometry showed a concentration-dependent and time-dependent increase, indicating that TK6 cells could uptake both types of nanoparticles. In the comet assay, AgNPs could induce a concentration-dependent increase in DNA tail intensity. However, titanium dioxide NPs could not induce the concentration-dependent increase of DNA fluorescence intensity of comet tail. In the PIG-A assay, both AgNPs and TiO₂NPs did not induce PIG-A gene mutation frequency in TK6 cells. The results showed that AgNPs could induce DNA damage in TK6 cells, but could not induce increase of PIG-A gene mutation frequency. TiO₂NPs neither induce DNA damage in TK6 cells nor increase PIG-A mutation frequency. Further tests are needed to determine whether TiO₂NPs are genotoxic.

Key words: silver nanoparticles, titanium dioxide nanoparticles, comet assay, in vitro PIG-A assay, genotoxicity

Haimei Zhu, Pengcheng Huang, Tiantian Zhao, Changhui Zhou, Ruowan Li, Chunrong Yu, Zhiyong Chen, Linfeng Gu, Yan Chang. In vitro genotoxicity study of silver nanoparticles and titanium dioxide nanoparticles[J]. Hereditas(Beijing), 2020, 42(12): 1192-1200.

Figures/Tables 6

Table 1

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纳米物质	纯度	粒径范围(nm)	比表面积(m²/g)	振实密度(g/cm³)	外观描述	受试浓度(μmol/L)
AgNPs	＞99.9%	60~80	≥3	≥2.5	黑色粉末	20、60、200
TiO₂NPs	99%	15~25	77.37	-	白色蓬松粉末	20、60、200

In vitro genotoxicity study of silver nanoparticles and titanium dioxide nanoparticles

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