通过时间基因表达谱分析探究异烟肼引起肝损伤的机制

doi:10.16288/j.yczz.22-069

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Hereditas(Beijing) ›› 2022, Vol. 44 ›› Issue (6): 501-509.doi: 10.16288/j.yczz.22-069

• Research Article • Previous Articles Next Articles

Analysis of time-series gene expression data to explore mechanisms of isoniazid-induced liver toxicity

Zizhao Tian(), Chenxi Zhou, Wei Zhou, Mo Li, Yunpeng Chu, Cong Huai(), Shengying Qin()

Bio-X Institute, Shanghai Jiaotong University, Shanghai 200030, China

Received:2022-03-10 Revised:2022-04-26 Online:2022-06-20 Published:2022-05-11
Contact: Huai Cong,Qin Shengying E-mail:tianzizhao@sjtu.edu.cn;huaic@sjtu.edu.cn;chinsir@sjtu.edu.cn
Supported by:
Supported by the National Nature Science Foundation of China Nos(81773818);Supported by the National Nature Science Foundation of China Nos(82003856)

Abstract

Abstract:

Isoniazid (INH) is a first-line anti-tuberculosis drug which can cause idiosyncratic liver injury, while the underlying mechanisms need to be further elucidated. In this study, we explored the time series gene expression profiling of a hepatocyte cell line under isoniazid treatment. Through cluster analysis and enrichment analysis of differentially expressed genes, we revealed a total of 6 gene clusters and a series of pathways related to hepatotoxicity, and 13 key candidate genes were identified according to the protein-protein interaction (PPI) network analysis and maSigPro analysis. These findings lay a foundation for understanding the mechanisms of isoniazid -induced liver toxicity and provide new target genes for the monitoring and treatment of INH-induced hepatotoxicity in the future.

Key words: isoniazid, hepatocytes, liver toxicity, time series analysis

Zizhao Tian, Chenxi Zhou, Wei Zhou, Mo Li, Yunpeng Chu, Cong Huai, Shengying Qin. Analysis of time-series gene expression data to explore mechanisms of isoniazid-induced liver toxicity[J]. Hereditas(Beijing), 2022, 44(6): 501-509.

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样本名称	质控数据	Q30 (%)	GC (%)	比对率(%)	样本名称	质控数据	Q30 (%)	GC (%)	比对率(%)
6h-1	32847297	95.14	53.39	96.27	18h-1	26368961	94.78	53.41	95.71
6h-2	34042755	95.16	53.32	96.08	18h-2	29140435	94.5	53.4	95.52
6h-3	34027030	94.68	53.61	96.30	18h-3	33440673	95.32	53.24	95.65
12h-1	30967636	94.71	53.46	95.77	24h-1	30981366	95.59	53.61	96.28
12h-2	30948041	94.73	53.34	96.02	24h-2	29293294	94.83	53.81	93.50

组合	12h vs 6h	18h vs 12h	24h vs 18h
上调基因数目	415	195	643
下调基因数目	180	84	928
合计	595	279	1571

基因名称	所属 Cluster
KAT6A、ARID1A、BCOR	Cluster1
TP53BP1、BUB1B	Cluster3
SRCAP	Cluster4
MRPL27、MRPL39、MALSU1、MRPL17、MRPL36、MRPS18B、MRPL46	Cluster6

Analysis of time-series gene expression data to explore mechanisms of isoniazid-induced liver toxicity

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