链霉菌较小基因组构建的研究进展

doi:10.16288/j.yczz.25-008

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Hereditas(Beijing) ›› 2025, Vol. 47 ›› Issue (7): 756-767.doi: 10.16288/j.yczz.25-008

• Review • Previous Articles Next Articles

Progress on the construction of Streptomyces minimized genomes

Hui Li(), Lijuan Guo, Hexia Zhou, Chunyang Li, Jialin Dai, Lizhu Shen, Rui Zhang, Fenghui Sun()

School of Laboratory Medicine, Chengdu Medical College, Chengdu 610500, China

Received:2025-03-01 Revised:2025-04-21 Online:2025-07-20 Published:2025-04-28
Contact: Fenghui Sun E-mail:huili@cmc.edu.cn;sunfenghui@cmc.edu.cn
Supported by:
School-level Fund of Chengdu Medical College(CYZYB24-07);Open Fund of Sichuan Provincial Engineering Laboratory for Prevention and Control Technology of Veterinary Drug Residue in Animal-origin(24LHGCSYS1-04);Open Fund of Sichuan Provincial Engineering Laboratory for Prevention and Control Technology of Veterinary Drug Residue in Animal-origin(24LHGCSYS1-06);Open Fund of Development and Regeneration Key Laboratory of Sichuan Province(24LHFYSZ1-48)

Abstract

Abstract:

The genus Streptomyces provides a wealth of natural products with diverse structures, accounting for more than two-thirds of natural antibiotics. However, the original Streptomyces producers of desired natural products are technically challenging due to their harsh cultivation conditions, slow growth rate and intricate genetic backgrounds. Meanwhile, the metabolites of Streptomyces can also cause interference with the extraction of the target natural products. Thus, there is an urgent need to develop an ideal Streptomyces chassis with characterization of clean metabolic background, fluent genetic manipulation, and higher success rate of biosynthetic gene cluster expressions, which can largely increase yield and reduce production costs of natural products. In this review, we summarize the recent development of the chassis in Streptomyces and its important roles in the excavation and production of natural products, with focus on its potential for discovery and synthesis of natural products.

Key words: Streptomyces, minimal genome, natural products

Hui Li, Lijuan Guo, Hexia Zhou, Chunyang Li, Jialin Dai, Lizhu Shen, Rui Zhang, Fenghui Sun. Progress on the construction of Streptomyces minimized genomes[J]. Hereditas(Beijing), 2025, 47(7): 756-767.

Figures/Tables 3

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菌株	底盘	野生型(Mb)	敲除大小(Mb)	底盘细胞基因组(Mb)	比例(%)	参考文献
阿维链霉菌(S. avermitilis)	SUK3	9.02	1.51	7.53	16.74	[56]
阿维链霉菌(S. avermitilis)	SUK17	9.02	1.67	7.35	18.51	[56]
天蓝色链霉菌(S. coelicolor)	ZM12	8.66	1.22	7.44	14.08	[25]
	M1146	8.66	0.17	8.49	1.96	[57]
	M1152	8.66	0.17	8.49	1.96	[57]
	M1154	8.66	0.17	8.49	1.96	[57]
白色链霉菌(S. albus)	Del14	6.80	0.50	6.33	7.35	[58]
变铅链霉菌(S. lividans)	ΔYA11	8.34	0.23	8.11	2.75	[59]

Progress on the construction of Streptomyces minimized genomes

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