遗传 ›› 2006, Vol. 28 ›› Issue (6): 737-744.

• 专论与综述 • 上一篇    下一篇

水稻单核苷酸多态性及其应用现状

刘传光1;张桂权2   

  1. 1. 广东省农业科学院水稻所,广州 510640;2. 华南农业大学农学院,广州 510642

  • 收稿日期:2005-11-14 修回日期:2006-01-18 出版日期:2006-06-10 发布日期:2006-06-10
  • 通讯作者: 张桂权

Single Nucleotide Polymorphism (SNP) and Its Application in Rice

LIU Chuan-Guang1;ZHANG Gui-Quan2   

  1. (1. Guangdong Academy of Agricultural Sciences, Guangzhou 510640,China;
    (2. College of Agronomy, South China Agricultural University, Guangzhou 510642, China

  • Received:2005-11-14 Revised:2006-01-18 Online:2006-06-10 Published:2006-06-10
  • Contact: ZHANG Gui-Quan

摘要: 单核苷酸多态性(single nucleotide polymorphisms, SNPs)在水稻中数量多,分布密度高,遗传稳定性高。水稻SNPs的发现方法主要有对样本DNA的PCR产物直接测序、从SSR区段检测SNPs和从基因组序列直接搜索等。目前已有多种基因分型技术运用到了水稻SNPs检测,SNPs检测的高度自动化使水稻SNPs基因分型非常方便。单核苷酸多态性在水稻遗传图谱的构建、基因克隆和功能基因组学研究、标记辅助选择育种、遗传资源分类及物种进化等方面的应用具有巨大潜力。

关键词: 单核苷酸多态性, 水稻, 基因分型, 遗传育种

Abstract: Single nucleotide polymorphisms (SNPs) distribute numerously and high-density throughout the rice genome and are stable in genetics. Several routes have been used for discover SNPs in rice such as sequencing directly to the PCR products of the DNA samples, screening SNPs in the SSR areas and search for SNPs through the rice genome sequences . several genotyping systems have been developed to identify SNPs in the rice genome. High automation in the SNPs identification has become a very convenient operation by the automatized systems. SNP has showed the huge potential in the establishing in the rice genetic maps, genes cloning and functional genomics, MAS in rice breeding and studying in classification and evolution of germplasm.

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