利用CRISPR/Cas9系统构建FGF21基因敲除小鼠模型
刘旭(
),张平,张晓枫,李兴,白宇,贾克荣,郭晓东,张豪,马晓燕,仓明,刘东军,郭旭东
),张平,张晓枫,李兴,白宇,贾克荣,郭晓东,张豪,马晓燕,仓明,刘东军,郭旭东
Construction of FGF21 knockout mouse models by the CRISPR/Cas9 system
Liu Xu(),Zhang Ping,Zhang Xiaofeng,Li Xing,Bai Yu,Jia Kerong,Guo Xiaodong,Zhang Hao,Ma Xiaoyan,Cang Ming,Liu Dongjun,Guo Xudong
),Zhang Ping,Zhang Xiaofeng,Li Xing,Bai Yu,Jia Kerong,Guo Xiaodong,Zhang Hao,Ma Xiaoyan,Cang Ming,Liu Dongjun,Guo Xudong
图3. Fgf21-/-小鼠FGF21 mRNA和FGF21蛋白表达情况 A：qRT-PCR检测FGF21 mRNA表达。M：DL500 bp;WT：野生型;KO：Fgf21-/-小鼠。B：Western blotting检测FGF21蛋白表达。WT：野生型小鼠;KO1、KO2、KO3：Fgf21-/-小鼠。
Fig. 3. FGF21 mRNA and FGF21 protein expression of Fgf21-/- by qRT-PCR and Western blotting
