敲降<i>VPS28</i>基因对中国荷斯坦奶牛乳脂合成的调控

敲降VPS28基因对中国荷斯坦奶牛乳脂合成的调控

刘莉莉,郭爱伟,吴培福,陈粉粉,杨亚晋,张勤

Regulation of VPS28 gene knockdown on the milk fat synthesis in Chinese Holstein dairy

Liu Lili,Guo Aiwei,Wu Peifu,Chen Fenfen,Yang Yajin,Zhang Qin

表2 候选基因的引物序列及其相对表达量

Table 2 Primers of the candidate genes for qRT-PCR and their relative expression

基因	引物序列 (5'→3')	相对表达量	基因	引物序列 (5'→3')	相对表达量
GAPDH	AGATGGTGAAGGTCGGAGTG	/	HERC3	CTCGAGGGCCTAGCTGTCT	0.73^*
GAPDH	CGTTCTCTGCCTTGACTGTG	/	HERC3	TTTGTCAGAAGGGTCTGGCG	0.73^*
VPS28	GGAAACAAGCCGGAGCTGTA	0.28^*	VPS45	CCCCAAAGATGCTGTGGCTA	0.42^*
VPS28	CTGGATCTCGTCCATGGCTC	0.28^*	VPS45	AGTGTGCTGGGGCCTAGATA	0.42^*
LPL	AGCTCCAAGTCGCCTTTCTC	0.48^*	CHMP2B	ACGAGGTACACAGAGGGCTA	0.38^*
LPL	TCCTGGTTGGAAAGTGCCTC	0.48^*	CHMP2B	AGCTGTTTGGCTAAAACTCTGC	0.38^*
LDLR	TGTTGGACACACGTACCCAG	2.72^*	CHMP3	GTTTGAAATCACCGCAGGGG	0.77^*
LDLR	AAGGTCGCGACTTGTCTCAG	2.72^*	CHMP3	CTAAAGGTTCAGGCTCCGGG	0.77^*
CD36	GACGGATGTACAGCGGTGAT	16.00^**	PIP5K	CTCAGCACCTGGAAGAGCAA	1.48^*
CD36	GAAAAAGTGCAAGGCCACCA	16.00^**	PIP5K	TTCTTCTTTCCCCGAGCCAC	1.48^*
ACSL1	GGGCCTGCGGAGGAGA	3.76^*	CYHR1	GCCAACCTGCTTTTGGGAAG	1.21^*
ACSL1	GGCAGCCGAAAGTACTGGAA	3.76^*	CYHR1	GGTTGTGAAAACGGCCACAA	1.21^*
FABP3	ACGCGTTCTCTGTCGTCTTT	3.79^*	CP	CATGGTGGCCAAAGGTGTTG	1.5^*
FABP3	AACCGACACCGAGTGACTTC	3.79^*	CP	CATCTGCTGGAGATTTTTGGCA	1.5^*
ACACA	AGTGTTCTGATCAGGTCTTCTTGT	0.67^*	PSMC1	GGTACGACTCCAACTCAGGC	2.90^*
ACACA	GGGAGGCAAAAACCTCCAGA	0.67^*	PSMC1	ATCCGGTTTGTGGCCATGAT	2.90^*
ACBP	TGGAATCTTTGCAACACCGC	0.83^*	PSMC3	TGAACAAGACGCTGCCGTAT	1.76^*
ACBP	TGTCACCCACAGTTGCTTGT	0.83^*	PSMC3	TGCCGCGTAGAGGTTTTGAT	1.76^*
FASN	AGGCGTGCGTGACACTT	6.85^*	PSMC5	CTCTGCACAAGATCCTGCCT	3.27^*
FASN	AATACAGTTGGCCGTCACCA	6.85^*	PSMC5	ATGCTTCACAGGCAGCTCAA	3.27^*
SCD	TCCTGATCATTGGCAACACCA	1.48^*	PSMD12	ATACGTCAGGCATCTCGCAG	0.37^*
SCD	CCAACCCACGTGAGAGAAGAA	1.48^*	PSMD12	GGCCATGTTGTAGGGGACAA	0.37^*
DGAT1	TACCCCGACAACCTGACCTA	2.06^*	UBB	TGGCATTGTTGGGTTCCTGT	0.56^*
DGAT1	GGGAAGTTGAGCTCGTAGCA	2.06^*	UBB	CGAAGATCTGCATTTTGACCTG	0.56^*
LPIN1	CTTCGATTCCCAAACCGGGA	2.35^*	UBC	GACCGGGAGTTCAGTCTTCG	1.28^*
LPIN1	TCACAGTGACGAACACCTGG	2.35^*	UBC	TTTACCAGTGAGGGTCTTCACAA	1.28^*
ADFP	GCGTCTGCTGGCTGATTTC	2.95^*	UBA52	GCCCAGTGACACCATTGAGA	1.28^*
ADFP	AGCCGAGGAGACCAGATCATA	2.95^*	UBA52	GCAGGGTGGACTCTTTCTGG	1.28^*
APOE	CATGCTGGGCCAGTCTACC	0.48^*	UBE2	CTGGCACAGTATATGAAGACCTGA	1.09^*
APOE	CTTCTTCAGGTCGTCAGCGTTGGAA	0.48^*	UBE2	GGTAGCAGGGTGTGAGGAAC	1.09^*
PRKCA	GACTTCGGGATGTGCAAGGA	2.40^*	TUBA	TGTCTACTCCTGTTGCCTGC	0.5^*
PRKCA	CGTACGGCTGATAGGCGATT	2.40^*	TUBA	AGGCATTGCCGATCTGGAC	0.5^*
MAPK1	AACAAAGTCCGAGTCGCCAT	2.54^*	ISG15	CCATCCTGGTGAGGAACGAC	19^**
MAPK1	CGATGGTCGGTGCTCGAATA	2.54^*	ISG15	GTCTGCTTGTACACGCTCCT	19^**
ARF6	AACTGGTATGTGTCAGCCCTC	4.68^*	MX1	TGCCAACTAGTCAGCACTACATT	1.08^*
ARF6	GAAAGAGGTGATGGTGGCGA	4.68^*	MX1	TGTACAGGTTGCTCTTGGACTC	1.08^*
STAM1	CCTGGTACTGCGGCTAACAA	1.23^*	SPP1	TCCGCCCTTCCAGTTAAACC	3.2^*
STAM1	ACGAACTTTCCGGCCTTCAT	1.23^*	SPP1	GCTTCTGAGATGGGTCAGGC	3.2^*
EEA1	CAGGCCCAGGACAGCTTAAA	7.68^*	RPS27A	TTTCGTGAAGACCCTGACGG	2.13^*
EEA1	GCAAGTTCCTGTGCTGCTTG	7.68^*	RPS27A	GTCTTTGCTGGTCAGGAGGAA	2.13^*