基于CSSLs群体定位和图位克隆水稻长芒基因GAD1-2

杨德卫,郑向华,程朝平,叶宁,黄凤凰,叶新福

Mapping and cloning of GAD1-2 for long awn using CSSLs in rice (Oryza sativa L.)

Yang Dewei,Zheng Xianghua,Cheng Chaoping,Ye Ning,Huang Fenghuang,Ye Xinfu

表1 本研究开发的SSR和Indel分子标记引物序列

Table 1 Indel and SSR molecular markers used in this study

标记 引物序列(5′→3′) 位点 扩增片段大小(bp) CM-2 F: TGGATGCGGGAGAGGTTGTCG P0461F06 196 R: TTCTATCACTTTGCCGGCCTAATCG CM-4 F: GGAGGAGAGCCAAGCGATGG P0013B04 111 R: ACCGTCTTGACGCTGAGAGTGC CM-5 F: CGACGAGCAAGTAGAGCACACG P0451G12 157 R: AGAATCTGACGACTGTGGGAACC CM-9 F: CATCCTACCCTATGACATGAGACC OJ1111_E05 110 R: CTCTAAGATTAGCGTTCCAGAGAGC CM-11 F: GCTTCGATTTAGCTATTGGTACGG OJ1666_A04 94 R: GTGCCAGCATGAAGAGGTATGG CM-61 F: CAGCTAGTGCCGGAAAGATTCG OJ1113_A10 200 R: CCCATGCAGTATATTTCGGTTGG CM-65 F: GTTCTTGAGGCACTCCCGATACC OJ1113_A10 292 R: CTTAAGCTCGCCAAGAACACACC CM-14 F: GCAGGTGCAATTTACTCATAGGG OSJNBb0092C08 249 R: GGATGATGATGACGACGATTACG CM-17 F: CGTCGTCCTCCGATCAAATCC OJ1111_H02 375 R: CTGGCTCGGTGTTCCAGTCG CM-21 F: CAGTCCAACGGGTTCGTCATCG P0481F05 392 R: TACCTGCAGTGCCTCCTCCAGTCC CM-26 F: ATGATTGCATCTGCATCACTGC P0481F05 379 R: ATACCTGTTTCCAATGCGTAGCC CM-29 F: CGGTCGGAGGTGTACACGAAGC P0028A08 236 R: GAGGAGATCCGGTGGGATTGC CM-68 F: CGATCAGATCTCCGCAAGTTGG P0605H02 361 R: GGCGTGGTTGATGGTGAACG CM-71 F: TGTCAGTGAGGTAGGTCAATAGC P0605H02 200 R: AGGCCCTAAGTCATATCTTCC CM-32 F: GATGGAGCCAAGACACGAAAGC P0686H11 127 R: TGACTTCGTCCGTATGCTACTGC CM-75 F: TTGCTACACCTTAGCTGCTGTGC P0686H11 300 R: GGTGTTCTTGTTGCTAGGAGATGC CM-35 F: TCCTCTCGTCATCTCACCTCACC B1142B04 178 R: CTCACCCACTCCTGTGTGACTCC CM-39 F: CTCTCCTCCTCACCTACGCATCC OJ1118_A06 289 R: GAAGTCCGGCTGGGAGTAGTGC CM-42 F: TGCAGAAGAACTACTGAGAGAAGACG OJ1345_D02 135 R: CATCTCCTTCAACCTGCCTTCC CM-77 F: AGGTTGACCTGTGTGAGTAGCAAGG OJ1345_D02 294 R: ACATCGCCAACCATCTCAAGG 标记 引物序列(5′→3′) 位点 扩增片段大小(bp) CM-46 F: CAATAAATCTCGCCCTCGTTGC OJ1134_H03 333 R: GTGAGGTCGGCCTTGAAGTACC CM-47 F: CCATCTCAACTCCTTCGTTTACTGC OSJNBa0025J22 297 R: TCGACTGTTTGCTTGGAATAGGC CM-51 F: GTCGGTCACGAAGTTCAGATCC B1168A08 186 R: TCAGGCAAAGTTGAAGATGGTAGC CM-52 F: CGGCGAGGTAGAAGGTGACG OSJNBa0016N23 196 R: CAGTGATTGTGTGACAGTGTGAGAGG CM-58 F: TCGATGGAGGAGGAGGAGTACG P0711H09 100 R: AAATTGATCGCTCCTCCACTGC Indel-3 F: CAGATGGCGAGTTGTCAGTTGC P0419H09 232 R: CATCTCGCCGATCCAAGTAAGC Indel-5 F: ACAGCCTATAGCTCACACCAAACC P0419H09 224 R: GAACACCTCCGTCTCCATTGC Indel-8 F: GACGCTGTAGAGACACAGATACATGG P0481F05 397 R: CTGTTGGTGATGGCCTTAGTGC Indel-11 F: AAGCTGCAGTTGGGTGAGAAGC P0481F05 484 R: GCCCAAGATACAGCAAGTTCTCG