Cas9蛋白变体VQR高效识别水稻NGAC前间区序列邻近基序
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辛高伟,胡熙璕,王克剑,王兴春
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Cas9 protein variant VQR recognizes NGAC protospacer adjacent motif in rice
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Xin Gaowei,Hu Xixun,Wang Kejian,Wang Xingchun
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| 表3 靶位点突变类型 |
| Table 3 Mutation type of target sites |
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| 靶位点 | 突变率(%) | 杂合突变 | 双等位突变 | 嵌合体 | 纯合突变 | | 植株数 | 所占比例(%) | 植株数 | 所占比例(%) | 植株数 | 所占比例(%) | 植株数 | 所占比例(%) | | NAL1-Q1 | 9.75 | 3 | 75.00 | 0 | 0 | 1 | 25.00 | 0 | 0 | | NAL1-Q2 | 43.90 | 15 | 83.33 | 3 | 16.66 | 0 | 0 | 0 | 0 | | LPA1-Q | 29.26 | 6 | 50.00 | 5 | 41.66 | 1 | 8.33 | 0 | 0 | | NAL1-C | 47.36 | 19 | 70.37 | 6 | 22.22 | 1 | 3.70 | 1 | 3.70 | | GL1-C | 77.19 | 19 | 43.18 | 12 | 27.27 | 11 | 25.00 | 2 | 4.54 |
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