中华蜜蜂酪胺受体基因克隆及表达分析

张丽珍,张永,胡景华,王子龙,曾志将

Molecular cloning and expression analysis of the tyramine receptor genes in Apis cerana cerana

Zhang Lizhen,Zhang Yong,Hu Jinghua,Wang Zilong,Zeng Zhijiang

表1 引物序列信息

Table 1 Primers used in this study

用途 基因名称 引物序列(5°→3°) PCR Actyr1 F:GCCAAACCAACCAGCAAATC R:CAATGGAGCGGGGGTAAAG Actyr2 F:AACAGCAACGGGATGAAGAA R: AGAAACGCACCCTGAAGAGAGT qRT-PCR Actyr1 F:AATCCAAGGAGTGCGAGGTG R:TGCGTGTCAATGGGAAGAAG Actyr2 F:TCTCTTCAGGGTGCGTTTCT R:GTTAACCTGCGACGTGCTCT