组蛋白H3变体H3.3及其在细胞重编程中的作用

黄星卫,程香荣,王楠,张雨薇,廖辰,金连弘,雷蕾

Histone variant H3.3 and its functions in reprogramming

Huang Xingwei,Cheng Xiangrong,Wang Nan,Zhang Yuwei,Liao Chen,Jin Lianhong,Lei Lei

图3 SCNT和iPSC两种体细胞重编程过程中H3.3的变化情况 SCNT重编程过程中卵母细胞含有大量的母源H3.3,会替换体细胞核中原有的H3.3,而iPSC重编程过程中没有母源H3.3参与。如果进行连续iPSC重编程,从第二代iPSC起可检测到LINEs和LTRs的缺失,到第六代时,四倍体补偿的iPSC胎儿会因非同义的SNVs累积致病基因而死亡[65]。SCNT：体细胞核移植(somatic cell nuclear transfer);iPSC：诱导多潜能干细胞(induced pluripotent stem cell);SNVs：单核苷酸突变(single-nucleotide variations);LINE：长散在核元件(long interspersed nucleotide elements);LTRs：长末端重复序列(long terminal repeats)。

Fig. 3 Comparison of H3.3 in somatic cell reprogramming during SCNT and iPSC