Ash2l-1/Ash2l-2在小鼠胚胎干细胞中的表达特异性及互补效应

谢晶,范辰,张景龙,张仕强

Expression specificity and compensation effect of Ash2l-1/Ash2l-2 in mouse embryonic stem cells

Xie Jing,Fan Chen,Zhang Jinglong,Zhang Shiqiang

表1 靶向Ash2l-1-CpG岛和Ash2l-2-RLTR12B的gRNAs序列信息

Table 1 Sequences of gRNAs targeting Ash2l-1-CpG islands and Ash2l-2-RLTR12B

名称 序列(5′→3′) PAM Tm(℃) GC(%) Ash2l-1-CpG岛-5′-gRNA-1 ACTTTGAGTCCGCCGCTTTA AGG 58 50 Ash2l-1-CpG岛-5′-gRNA-2 AAAACGCCTGCTCATAGTCG TGG 57 50 Ash2l-1-CpG岛-3′-gRNA-1 TGCACTGGGCTAACGACGGG AGG 63 65 Ash2l-1-CpG岛-3′-gRNA-2 GACAAATGTGTTCGCGGCCC AGG 61 60 Ash2l-2-RLTR12B-5′gRNA-1 CTAATTACTGCGACAGTTGC AGG 53 45 Ash2l-2-RLTR12B-5′-gRNA-2 GAGAACCGCGATAGCAGTCC AGG 59 60 Ash2l-2-RLTR12B-3′-gRNA-1 AAATGCGTGTGCGCGCGCTG GGG 67 65 Ash2l-2-RLTR12B-3′-gRNA-2 TGCCAGCAAACCCGAGGATC TGG 61 60