Ash2l-1/Ash2l-2在小鼠胚胎干细胞中的表达特异性及互补效应
|
|
谢晶,范辰,张景龙,张仕强
|
Expression specificity and compensation effect of Ash2l-1/Ash2l-2 in mouse embryonic stem cells
|
|
Xie Jing,Fan Chen,Zhang Jinglong,Zhang Shiqiang
|
|
| 表3 PCR鉴定引物序列信息 |
| Table 3 Primer sequences for PCR validation |
|
| 引物名称 | 引物序列(5′→3′) | Tm(℃) | GC(%) | | Ash2l-1-CpG岛-1 | F: AGTGTCTCCGGTACCTTCCT | 58 | 55 | | R: GTGTGAGCCAAGAGCGTTTC | 58 | 55 | | Ash2l-1-CpG岛-2 | F: CCTTACCCACTTTCGGCCTC | 59 | 60 | | R: TGCTCCGCACAGTGTAAAGG | 59 | 55 | | Ash2l-1-CpG岛-3 | F: CCCACACTGCAACTCCACTTCTCCCAACTGCCGCCCAT | 75 | 61 | | R: CCACCCACAACTACCTCGGGCTCTCTGGAATCAGACCT | 72 | 58 | | Ash2l-2-RLTR12B-1 | F: GTCCCCCACTGACATGGTTT | 58 | 55 | | R: TCAAGGGCTGCGAATTGTCT | 58 | 50 | | Ash2l-2-RLTR12B-2 | F: CCTTAGACCTTGCTTGGTGACA | 58 | 50 | | R: AGCAAGAATGGCTCATGGTAGA | 57 | 45 |
|
|
|