Ash2l-1/Ash2l-2在小鼠胚胎干细胞中的表达特异性及互补效应

谢晶,范辰,张景龙,张仕强

Expression specificity and compensation effect of Ash2l-1/Ash2l-2 in mouse embryonic stem cells

Xie Jing,Fan Chen,Zhang Jinglong,Zhang Shiqiang

表4 CRISPR/Cas9组合3和组合4的敲除效率

Table 4 Knockout efficiency of CRISPR/Cas9 combination 3 and 4

敲除靶点 克隆总数 野生型(+/+) 单等位基因敲除(+/-) 双等位基因敲除(-/-) Ash2l-1-CpG岛 48 26(54.1%) 14(29.2%) 8(16.7%) Ash2l-2-RLTR12B 32 22(68.7%) 7(21.9%) 3 (9.4%)