鸡快慢羽性状遗传学基础分析综合实验探索与实践

张丽,徐海冬,冷奇颖,刘艳芬,赵志辉,效梅,吴江,张权,安立龙

A genetics laboratory class to analyze early and late feather traits of chicken

Zhang Li,Xu Haidong,Leng Qiying,Liu Yanfen,Zhao Zhihui,Xiao Mei,Wu Jiang,Zhang Quan,An Lilong

表1 快慢羽基因分型引物

Table 1 The primers to genotype the early and late feather traits

引物名称 序列(5°→3°) 用途 Tm(℃) PR-F AATGGTACTACAGAGAAGGTAGGAATATC 双重PCR快慢羽分型 58 PR-R CCTAGAACACTGGACATGGTATGATATCTCAGCC PR-in GGTGTGCACCTGGGTGTAGTGGACA URev21-F(PR-F) AATGGTACTACAGAGAAGGTAGGAATATC 纯合子、杂合子分析 56 URev21-R GTAAGACTAACACAGTATTCTCGAGT