CRISPR/Cas9系统在昆虫中的应用

童晓玲,方春燕,盖停停,石津,鲁成,代方银

Applications of the CRISPR/Cas9 system in insects

Tong Xiaoling,Fang Chunyan,Gai Tingting,Shi Jin,Lu Cheng,Dai Fangyin

表1 CRISPR/Cas9在昆虫中的应用

Table 1 The applications of CRISPR/Cas9 in insects

昆虫 基因 导入策略 突变形式 参考文献 黑腹果蝇 (Drosophila melanogaster) Yellow, white mRNA Knock-out [38] Yellow, rosy Plasmid Knock-out, Knock-in [14] Ast, capa, Ccap, Crz, Eh, Mip, npf, miR-219, miR-315, White Transgene, plasmid Knock-out [15] White Transgene, plasmid Knock-out [11] Phosphoglycerate kinase Plasmid Knock-out [13] Ebony, yellow, white Cas9-transgene, plasmid Knock-out, Knock-in [39] Sosy, DSH3PX1 Cas9-transgene, plasmid Knock-out, Knock-in [40] Nicotinic acetylcholine receptor dCas9-VPR-transgene, plasmid Transcriptional activation [41] Ebony, Singed Cas9-protein, mRNA Knock-out [42] Piwi Cas9D10A-transgene, plasmid Knock-out, Knock-in [43] Ebony, vermillion, yellow, white Cas9-transgene, plasmid Knock-out [20] EGFP, Mrfp Cas9-transgene, plasmid Knock-out [21] Ms(3)k81, white, yellow Cas9-transgene, plasmid Knock-out, Knock-in [44] Bam, nos, yellow, cid, notch CRISPR/Cas9 conditional mutagenesis, transgene Knock-out [45] Ebony, sepia, forked, curled, wnt Cas9-transgene, plasmid Knock-out [19] Dα6, G275E Plasmid Knock-out, Knock-in [46] AatII, M4790I Cas9-transgene, plasmid Knock-in [47] Wg, hnt, cut, scute dCas9-VPR-transgene Cas9 transcriptional activators [48] Ebony, lbk nos-Cas9-transgene, plasmid Co-CRISPR-knock-out [49] WingGFP-tan Plasmid scarless allele replacement [50] 斑翅果蝇 (D. suzukii) White, Sex Lethal Plasmid Knock-out [51] 埃及伊蚊 (Aedes aegypti) miR-309 mRNA Knock-out [52] Nix Cas9-transgene, plasmid Gene Drive [53] Nix Cas9-protein, mRNA Knock-out [37] ECFP mRNA Knock-out [54] Kmo, lig4, ku70, r2d2, lops Cas9-protein, mRNA Knock-out [57] 致乏库蚊 (Culex quinquefasciatus) CYP9M10 mRNA Knock-out [55] 冈比亚按蚊 (Anopheles gambiae) AGAP005958, AGAP011377, AGAP007280) Plasmid Gene drive, Knock-out, Knock-in [26] 史按蚊 (Anopheles stephensi) Hydroxylasewhite Plasmid Gene drive, Knock-in [56] 家蚕 (Bombyx mori) BmBLOS2 mRNA Knock-out [22] Kynu-2, yellow-e, ebony, BmBlos2, tyrosine hydroxylase, red egg, flugellos Cas9-protein, mRNA, plasmid Knock-out [24] Bmku70 Plasmid Knock-out [25] Bm-ok, BmKMO, BmTH, Bmtan mRNA Knock-out [57] BmBLOS2, EGFP Plasmid Knock-out [32] BmBlos2, BmP25, BmSericin1, BmSericin3 SpCas9/SaCas9/AsCpf-protein, plasmid, mRNA Knock-out [33] 昆虫 基因 导入策略 突变形式 参考文献 柑橘凤蝶 (Papilio xuthus) Abdominal-B, ebony, frizzled mRNA Knock-out [29] 小红蛱蝶 (Vanessa cardui) Spalt, distal-less Cas9-protein, mRNA Knock-out [58] 鹿眼蛱蝶 (Junonia coenia) Spalt, distal-less Cas9-protein, mRNA Knock-out [31] 小菜蛾 (Plutella xylostella) Abdominal-A mRNA Knock-out [59] 斜纹夜蛾 (Spodoptera litura) SlitPBP3 mRNA Knock-out [60] Slabd-A mRNA Knock-out [61] 棉铃虫 (Helicoverpa armigera) Cadherin mRNA Knock-out [62] HarmPBP1 mRNA Knock-out [63] HaABCA2 Cas9-protein, mRNA Knock-out [64] HarmOR16 mRNA Knock-out [65] White, Brown, Scarlet, Ok mRNA Knock-out [66] 棉叶虫 (Spodoptera littoralis) SlitOrco Cas9-protein, mRNA Knock-out [67] 赤拟谷盗 (Tribolium castaneum) EGFP Plasmid, mRNA Knock-out, Knock-in [68] W, Ace2, TC010993 Plasmid Gene drive [69] Robo2/3 nos-Cas9-transgene, plasmid Knock-out, Knock-in [70] 飞蝗 (Locusta migratoria) Orco mRNA Knock-out [71] 双斑蟋 (Gryllus bimaculatus) Dop1 mRNA Knock-out [72]