谷子MYB类转录因子SiMYB42提高转基因拟南芥低氮胁迫耐性

丁庆倩,王小婷,胡利琴,齐欣,葛林豪,徐伟亚,徐兆师,周永斌,贾冠清,刁现民,闵东红,马有志,陈明

MYB-like transcription factor SiMYB42 from foxtail millet (Setaria italica L.) enhances Arabidopsis tolerance to low-nitrogen stress

Ding Qingqian,Wang Xiaoting,Hu Liqin,Qi Xin,Ge Linhao,XU Weiya,Xu Zhaoshi,Zhou Yongbin,Jia Guanqing,Diao Xianmin,Min Donghong,Ma Youzhi,Chen Ming

表1 本研究所用的引物信息

Table 1 Primers information for this study

引物名称 引物序列(5′→3′) 用途 复性温度(℃) SiMYB42 F:ATGGGTTCTACAACGGCGA 基因克隆 59 SiMYB42 R:TCATCGCTTCCCTATAGCATTA SiMYB42-pBI121 F:CTCTAGAGGATCCCCGGGATTTGGGCTTCTCG pBI121载体构建 65 SiMYB42-pBI121 R:ACTAGTGGATCCCCCGGGTTCACCTGCTGCAGC SiMYB42-GFP F:ATCTCTAGAGGATCCATGGGGCGGCAGC GFP载体构建 60 SiMYB42-GFP R:GCTCACCATGGATCCGAACTTTGCTCCG SiMYB42-BD F:AGGAGGACCTGCATATGATGGGGCGGCAGCCGTGCT BD载体构建 68 SiMYB42-BD R:GCCTCCATGGCCATATGGAACTTTGCTCCGTTTGAG SiMYB42-RT F:TGGACGAGTTCAGCAGCAT 实时定量PCR 60 SiMYB42-RT R:CTCCGTTTGAGCCGTTGA SiActin F:GGCAAACAGGGAGAAGATGA 谷子内参基因扩增 60 SiActin R:GAGGTTGTCGGTAAGGTCACG