N-WASP通过polyPro和VCA结构域调控大脑皮层神经元迁移

沈秀莲,逯宜超,甲芝莲,吴强

N-WASP regulates cortical neuron migration through its polyPro and VCA domains

Shen Xiulian,Lu Yichao,Jia Zhilian,Wu Qiang

表1 本研究使用的引物

Table 1 Primers used in this study

引物 序列(5°→3°) N-WASP shRNA1-F CCGGAAGACGAGATGCTCCAAATGGCTCGAGCCATTTGGAGCATCTCGTCTTTTTTTG N-WASP shRNA1-R AATTCAAAAAAAGACGAGATGCTCCAAATGGCTCGAGCCATTTGGAGCATCTCGTCTT N-WASP shRNA2-F CCGGCAGATACGACAGGGCATTCAACTCGAGTTGAATGCCCTGTCGTATCTGTTTTTG N-WASP shRNA2-R AATTCAAAAACAGATACGACAGGGCATTCAACTCGAGTTGAATGCCCTGTCGTATCTG N-WASP_EcoRI_F TGGAATTCGACACCATGAGCTCGGGCCAGCAG N-WASP_HindIII_R CCCAAGCTTGCGTCTTCCCACTCATCATC N-WASP_H208D_F TCCAGGACATTGGGCATGTTG N-WASP_H208D_R CCAATGTCCTGGAAATTACTTGG N-WASP_ΔVCA_R CCCAAGCTTGCGTCACCATCAGAAGGCAGGC VCA_EcoRI_F TGGAATTCGACACCATGGACCATCAAGTTCCAGC WH1_HindIII_R CCCAAGCTTGCTAGATTGGGACCATTTGGAGCAT N-WASP_ΔWH1_F TGGAATTCGACACCATGCTACCCATGGCTACAGTTG N-WASP_ΔpolyPro_R CTTGATGGTCTGGTGCTTGTCTTCGGAGTTC N-WASP_ΔpolyPro_F ACAAGCACCAGACCATCAAGTTCCAGC polyPro_EcoRI_F TGGAATTCGACACCATGAGACAAGCACCACCACCACCT WH1_GBD_HindIII_R CCCAAGCTTGCTGGTGCTTGTCTTCGGAGTTC SCR_F CCGGCAACAAGATGAAGAGCACCAACTCGAGTTGGTGCTCTTCATCTTGTTGTTTTTG SCR_R AATTCAAAAACAACAAGATGAAGAGCACCAACTCGAGTTGGTGCTCTTCATCTTGTTG