利用CRISPR/Cas9双基因敲除系统初步解析大豆GmSnRK1.1和GmSnRK1.2对ABA及碱胁迫的响应

李慧卿,陈超,陈冉冉,宋雪薇,李佶娜,朱延明,丁晓东

Preliminary analysis of the role of GmSnRK1.1 and GmSnRK1.2 in the ABA and alkaline stress response of the soybean using the CRISPR/Cas9-based gene double-knockout system

Li Huiqing,Chen Chao,Chen Ranran,Song Xuewei,Li Jina,Zhu Yanming,Ding Xiaodong

表3 PCR引物序列

Table 3 The sequences of PCR primers used in this study

引物名称 引物序列(5′→3′) GmSnRK1.1 F:AACACTCGGCATTGGGTCCT R:GGATGACCGCAATTAAGGCA GmSnRK1.2 F:TATTCGCTGAACCCCTCTCT R:AGACTGAGCAGGAGGTAACAT GmSnRK1.3 F:GAGGTGGTGCTGGACTGGACA R:ATGAACAAGACTGAGCACGAG GmSnRK1.4 F:AAACTCTTGGCATTGGATCC R:TGTTGTCACTCCATCTCTTTC Bar F:CCTTATCTGGGAACTACTCACAC R:CACCATCGTCAACCACTACAT