利用CRISPR/Cas9双基因敲除系统初步解析大豆GmSnRK1.1和GmSnRK1.2对ABA及碱胁迫的响应

李慧卿,陈超,陈冉冉,宋雪薇,李佶娜,朱延明,丁晓东

Preliminary analysis of the role of GmSnRK1.1 and GmSnRK1.2 in the ABA and alkaline stress response of the soybean using the CRISPR/Cas9-based gene double-knockout system

Li Huiqing,Chen Chao,Chen Ranran,Song Xuewei,Li Jina,Zhu Yanming,Ding Xiaodong

表4 转基因毛状根突变率

Table 4 Determination of mutation rates in transgenic hairy roots

靶基因 转基因毛状根数 基因突变毛状根数 突变率(%) 双基因突变毛状根数 双基因突变率(%) GmSnRK1.1 35 27 77.1 17 48.6 GmSnRK1.2 35 24 68.6