利用CRISPR/Cas9双基因敲除系统初步解析大豆GmSnRK1.1和GmSnRK1.2对ABA及碱胁迫的响应

李慧卿,陈超,陈冉冉,宋雪薇,李佶娜,朱延明,丁晓东

Preliminary analysis of the role of GmSnRK1.1 and GmSnRK1.2 in the ABA and alkaline stress response of the soybean using the CRISPR/Cas9-based gene double-knockout system

Li Huiqing,Chen Chao,Chen Ranran,Song Xuewei,Li Jina,Zhu Yanming,Ding Xiaodong

图8 CRISPR/Cas9介导的大豆GmSnRK1.1和GmSnRK1.2双突变基因型多态性 A：GmSnRK1.1突变基因型多态性;B：GmSnRK1.2突变基因型多态性。红色虚线表示缺失碱基;红色字体表示增加碱基;下划线表示gRNA靶位点;加粗表示PAM序列。

Fig. 8 Polymorphic mutant genotypes of soybean GmSnRK1.1 and GmSnRK1.2 genes mediated by the CRISPR/Cas9 system