靶向miRNA前体不同类型sgRNA的丰度及特异性评估

刘海龙,谌阳,高杨,周玲,韩晓松,赵长志,杨高娟,陈毅龙,杨慧,谢胜松

Assessing abundance and specificity of different types of sgRNA targeting miRNA precursors

Liu Hailong,Shen Yang,Gao Yang,Zhou Ling,Han Xiaosong,Zhao Changzhi,Yang Gaojuan,Chen Yilong,Yang Hui,Xie Shengsong

表3 靶向miRNA前体不同类型sgRNA的数量及频率

Table 3 The number and frequency of different types of sgRNAs targeting miRNA precursors

靶向miRNA前体的sgRNA类型数量(个) 单个sgRNA靶点 成对gRNAs靶点 miRNA前体(个) 频率(%) miRNA前体(个) 频率(%) 0 21 0.07 6842 23.89 1 15 0.05 8523 29.75 2 31 0.11 6963 24.31 3 165 0.58 3863 13.49 4 833 2.91 1646 5.75 5 2079 7.26 593 2.07 6 4393 15.34 164 0.57 7 6480 22.62 35 0.12 8 6893 24.06 15 0.05 9 4983 17.40 1 0 10 2218 7.74 0 0 11 534 1.86 0 0