靶向miRNA前体不同类型sgRNA的丰度及特异性评估

刘海龙,谌阳,高杨,周玲,韩晓松,赵长志,杨高娟,陈毅龙,杨慧,谢胜松

Assessing abundance and specificity of different types of sgRNA targeting miRNA precursors

Liu Hailong,Shen Yang,Gao Yang,Zhou Ling,Han Xiaosong,Zhao Changzhi,Yang Gaojuan,Chen Yilong,Yang Hui,Xie Shengsong

表5 miRNA前体长度、GC%含量与不同类型sgRNA丰度相关性分析

Table 5 Correlation analysis of miRNA precursor with sequence length, GC content (%), and abundance of different types of sgRNA

PAM类型 CRISPR核酸酶 与miRNA前体长度的相关性 (Pearson相关系数) 与GC%含量的相关性 (Pearson相关系数) NGA SpCas9 VQR变异体 0.80 -0.08 NGAG SpCas9 EQR变异体 0.49 0.12 NGCG SpCas9 VRER变异体 0.25 0.30 NGG SpCas9和FnCas9 0.59 0.35 NGGNG St3Cas9 0.36 0.31 NNAGAAW St1Cas9 0.39 -0.15 NNGRRT SaCas9 0.58 -0.10 NNNNGATT NmCas9 0.39 -0.19 NNNRRT SaCas9 KKH变异体 0.83 -0.32 TTTN AsCpf1和LbCpf1 0.65 -0.38 YG FnCas9 RHA变异体 0.80 0.12