miR-362靶向ZNF644基因调控猪未成熟支持细胞的增殖和凋亡
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冉茂良,董莲花,翁波,曹蓉,彭馥芝,高虎,罗荟,陈斌
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miR-362 regulates the proliferation and apoptosis of porcine immature Sertoli cells through targeting the ZNF644 gene
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Ran Maoliang,Dong Lianhua,Weng Bo,Cao Rong,Peng Fuzhi,Gao Hu,Luo Hui,Chen Bin
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| 表1 引物序列信息 |
| Table 1 Primers used in this study |
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| 名称 | 引物序列(5′→3′) | | miR-362 | RT:GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCACTCA | | F:TCG GAATCC TTGGAACCTAGGTG | | R:ATCCAGTGCAGGGTCCGAGG | | ZNF644 | F:CATCCTGGAAGAGCCGATAG | | R:GAGGATGTTCCGAATGTTGG | | Bcl2 | F:GAGGATTGTGGCCTTCTTTG | | R:GCCGGTTCAGGTACTCAGTC | | BAX | F:TCCGGGGAGCAACCCA | | R:AAACCCTGAAGCAAAAGGGC | | Caspase-3 | F:TGGATGCTGCAAATCTCA | | R:TCCCACTGTCCGTCTCAA | | GAPDH | F:GTTTGTGATGGGCGTGAAC | | R:ATGGACCTGGGTCATGAGT | | U6 | F:AACGCTTCACGAATTTGCGT | | R:CGCTTCACGAATTTGCGTGTCAT |
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