miR-362靶向ZNF644基因调控猪未成熟支持细胞的增殖和凋亡
miR-362 regulates the proliferation and apoptosis of porcine immature Sertoli cells through targeting the ZNF644 gene
miR-362靶向ZNF644基因调控猪未成熟支持细胞的增殖和凋亡 |
| 冉茂良,董莲花,翁波,曹蓉,彭馥芝,高虎,罗荟,陈斌 |
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miR-362 regulates the proliferation and apoptosis of porcine immature Sertoli cells through targeting the ZNF644 gene |
| Ran Maoliang,Dong Lianhua,Weng Bo,Cao Rong,Peng Fuzhi,Gao Hu,Luo Hui,Chen Bin |
| 图3 psiCHECK2-ZNF644-3′UTR-WT和psiCHECK2-ZNF644-3′UTR-MT质粒验证及双荧光素酶检测结果 A：psiCHECK2-ZNF644-3′UTR-WT和psiCHECK2-ZNF644-3′UTR-MT质粒鉴定;B：采用XhoⅠ和NotⅠ内切酶对psiCHECK2-ZNF644- 3′UTR-WT和psiCHECK2-ZNF644-3′UTR-MT质粒进行双酶切鉴定;C：ST细胞中荧光素酶活性检测miR-362对ZNF644 3′UTR的调控。M：DL2000 marker;1：psiCHECK2-ZNF644-3′UTR-WT重组质粒;2：psiCHECK2-ZNF644-3′UTR-MT重组质粒;3：siCHECK2- ZNF644-3′UTR-MT重组质粒双酶切结果;4：psiCHECK2-ZNF644-3′UTR-WT重组质粒双酶切结果;*P<0.05表示差异显著。 |
| Fig. 3 Characterization of psiCHECK2-ZNF644-3′UTR-WT and psiCHECK2-ZNF644-3′UTR-MT plasmid and results of dual-luciferase assays |
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