绵羊高效转基因通用型piggyBac转座子载体构建及功能验证

胡广东,郝科兴,黄涛,曾维斌,谷新利,王静

Exploration and characterization of a universal piggyBac transposon vector for efficient transgene studies in sheep

Hu Guangdong,Hao Kexing,Huang Tao,Zeng Weibin,Gu Xinli,Wang Jing

表1 本文所用的引物序列信息

Table 1 Primers used in this study

名称 引物序列(3′→5′) 用途 片段大小 PPP F CTCGCTCGAGTAAAACGACGGCCAGTGAAT pTK-SPBase-ployA 表达框PCR扩增 2.2 kb PPP R ATCGGCGGCCGCCGCTTACAATTTACGCGTTAAC SP1 TGACTCACGCGGTCGTTATAGTTCA Tail-PCR检测 SP2 GTGACACTTACCGCATTGACAAGCA SP3 CGGATTCGCGCTATTTAGAAAGAG