绵羊高效转基因通用型piggyBac转座子载体构建及功能验证

胡广东,郝科兴,黄涛,曾维斌,谷新利,王静

Exploration and characterization of a universal piggyBac transposon vector for efficient transgene studies in sheep

Hu Guangdong,Hao Kexing,Huang Tao,Zeng Weibin,Gu Xinli,Wang Jing

图2 PBase密码子偏好性优化结果 A:PBase基因偏好性优化CAI (0.97)指数调整结果。优化后CAI指数为0.97 (绿),优化前CAI指数为0.58 (蓝)。B:PBase基因密码子偏好性调整结果。对98个氨基酸密码子进行优化(绿)。C:PBase基因GC含量调整结果。优化后GC含量为56.36% (绿),优化前为40.3% (蓝) 。

Fig. 2 Optimization results of the PBase codon bias