基于级联核酸侵入反应的real-time PCR法检测咽拭子样本中UGT1A1*6基因多态性

张婕妤,初亚男,邹秉杰,张晏洁,封利颖

Detection of UGT1A1*6 single nucleotide polymorphism in oral swap samples using the cascade invader assay-based real-time PCR

Zhang Jieyu,Chu Yanan,Zou Bingjie,Zhang Yanjie,Feng Liying

表1 基于级联核酸侵入反应的real-time PCR检测UGT1A1*6基因型的扩增引物和探针序列

Table 1 Sequences of amplification primers and probes detecting UGT1A1*6 genotype using cascade invader assay-based real-time PCR

引物名称 序列(5°→3°) 突变型荧光发卡探针FRET-1 FAM-TCTT(BHQ1)AGCCGGTTTTCCGGCTAAGACTCCGCGTCCGT-C6-NH2 野生型荧光发卡探针FRET-2 VIC-TCTT(BHQ1)AGCCGGTTTTCCGGCTAAGACCTCGGCGCG-C6-NH2 PCR正向引物 TGTCCTGCACCTGACGCCT PCR反向引物 ACATCCTCCCTTTGGAATGGCACA 侵入探针 GTACGTCTTCAAGGTGTAAAATGCTCA 野生型检测探针 CGCGCCGAGGCGTCTCTGATGTACAACG-PO3 突变型检测探针 ACGGACGCGGAGTGTCTCTGATGTACAACG-PO3