基于级联核酸侵入反应的real-time PCR法检测咽拭子样本中UGT1A1*6基因多态性

张婕妤,初亚男,邹秉杰,张晏洁,封利颖

Detection of UGT1A1*6 single nucleotide polymorphism in oral swap samples using the cascade invader assay-based real-time PCR

Zhang Jieyu,Chu Yanan,Zou Bingjie,Zhang Yanjie,Feng Liying

表2 焦测序检测UGT1A1*6基因型的扩增引物和测序引物序列

Table 2 Sequences of amplification and sequencing primers for detection UGT1A1*6 genotype using pyrosequencing

引物名称 序列(5°→3°) 正向生物素 修饰扩增引物 Biotin-CAGCAGAGGGGACATGAAATAG 反向扩增引物 CCTCCCTTTGGAATGGCAC 测序引物 GTCTTCAAGGTGTAAAATGCTC